8DLT

Cryo-EM structure of SARS-CoV-2 Epsilon (B.1.429) spike protein

これはPDB形式変換不可エントリーです。

8DLT の概要

• エントリーDOI: 10.2210/pdb8dlt/pdb
• EMDBエントリー: 27515
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: sars-cov-2, glycoprotein, fusion protein, viral protein, viral protein-immune system complex, epsilon, b.1.429
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 440017.57

構造登録者

Zhu, X.,Mannar, D.,Saville, J.W.,Srivastava, S.S.,Berezuk, A.M.,Zhou, S.,Tuttle, K.S.,Subramaniam, S. (登録日: 2022-07-08, 公開日: 2022-08-31, 最終更新日: 2024-11-13)

主引用文献

Mannar, D.,Saville, J.W.,Sun, Z.,Zhu, X.,Marti, M.M.,Srivastava, S.S.,Berezuk, A.M.,Zhou, S.,Tuttle, K.S.,Sobolewski, M.D.,Kim, A.,Treat, B.R.,Da Silva Castanha, P.M.,Jacobs, J.L.,Barratt-Boyes, S.M.,Mellors, J.W.,Dimitrov, D.S.,Li, W.,Subramaniam, S.
SARS-CoV-2 variants of concern: spike protein mutational analysis and epitope for broad neutralization.
Nat Commun, 13:4696-4696, 2022

PubMed Abstract: Mutations in the spike glycoproteins of SARS-CoV-2 variants of concern have independently been shown to enhance aspects of spike protein fitness. Here, we describe an antibody fragment (V ab6) that neutralizes all major variants including the recently emerged BA.1 and BA.2 Omicron subvariants, with a unique mode of binding revealed by cryo-EM studies. Further, we provide a comparative analysis of the mutational effects within previously emerged variant spikes and identify the structural role of mutations within the NTD and RBD in evading antibody neutralization. Our analysis shows that the highly mutated Gamma N-terminal domain exhibits considerable structural rearrangements, partially explaining its decreased neutralization by convalescent sera. Our results provide mechanistic insights into the structural, functional, and antigenic consequences of SARS-CoV-2 spike mutations and highlight a spike protein vulnerability that may be exploited to achieve broad protection against circulating variants.

PubMed: 35982054
DOI: 10.1038/s41467-022-32262-8

実験手法

ELECTRON MICROSCOPY (2.4 Å)