8DK1

CryoEM structure of JetABC (head construct) from Pseudomonas aeruginosa PA14

8DK1 の概要

• エントリーDOI: 10.2210/pdb8dk1/pdb
• EMDBエントリー: 25913 27480
• 分子名称: JetA, JetC, JetB (3 entities in total)
• 機能のキーワード: wadjet, bacterial defense systems, jeta, jetb, jetc, anti-plasmid defense system, epta, eptb, eptc, mksb, mkse, mksf, smc, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Pseudomonas aeruginosa PA14; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 369965.90

構造登録者

Deep, A.,Gu, Y.,Gao, Y.,Ego, K.,Herzik, M.,Zhou, H.,Corbett, K. (登録日: 2022-07-01, 公開日: 2022-10-05, 最終更新日: 2024-06-12)

主引用文献

Deep, A.,Gu, Y.,Gao, Y.Q.,Ego, K.M.,Herzik Jr., M.A.,Zhou, H.,Corbett, K.D.
The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.
Mol.Cell, 82:4145-, 2022

PubMed Abstract: Self versus non-self discrimination is a key element of innate and adaptive immunity across life. In bacteria, CRISPR-Cas and restriction-modification systems recognize non-self nucleic acids through their sequence and their methylation state, respectively. Here, we show that the Wadjet defense system recognizes DNA topology to protect its host against plasmid transformation. By combining cryoelectron microscopy with cross-linking mass spectrometry, we show that Wadjet forms a complex similar to the bacterial condensin complex MukBEF, with a novel nuclease subunit similar to a type II DNA topoisomerase. Wadjet specifically cleaves closed-circular DNA in a reaction requiring ATP hydrolysis by the structural maintenance of chromosome (SMC) ATPase subunit JetC, suggesting that the complex could use DNA loop extrusion to sense its substrate's topology, then specifically activate the nuclease subunit JetD to cleave plasmid DNA. Overall, our data reveal how bacteria have co-opted a DNA maintenance machine to specifically recognize and destroy foreign DNAs through topology sensing.

PubMed: 36206765
DOI: 10.1016/j.molcel.2022.09.008

実験手法

ELECTRON MICROSCOPY (2.95 Å)