8DHU

Crystal structure of LARP-DM15 from Drosophila melanogaster bound to m7GpppC

8DHU の概要

• エントリーDOI: 10.2210/pdb8dhu/pdb
• 分子名称: La-related protein 1, [[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-1~{H}-purin-9-ium-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [[(2~{R},3~{S},4~{R},5~{R})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: larp, dm15, rna binding protein
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37915.45

構造登録者

Nguyen, E.,Berman, A.J. (登録日: 2022-06-28, 公開日: 2024-01-17, 最終更新日: 2024-09-04)

主引用文献

Nguyen, E.,Sosa, J.A.,Cassidy, K.C.,Berman, A.J.
Comparative analysis of the LARP1 C-terminal DM15 region through Coelomate evolution.
Plos One, 19:e0308574-e0308574, 2024

PubMed Abstract: TOR (target of rapamycin), a ubiquitous protein kinase central to cellular homeostasis maintenance, fundamentally regulates ribosome biogenesis in part by its target La-related protein 1 (LARP1). Among other target transcripts, LARP1 specifically binds TOP (terminal oligopyrimidine) mRNAs encoding all 80 ribosomal proteins in a TOR-dependent manner through its C-terminal region containing the DM15 module. Though the functional implications of the LARP1 interaction with target mRNAs is controversial, it is clear that the TOP-LARP1-TOR axis is critical to cellular health in humans. Its existence and role in evolutionarily divergent animals remain less understood. We focused our work on expanding our knowledge of the first arm of the axis: the connection between LARP1-DM15 and the 5' TOP motif. We show that the overall DM15 architecture observed in humans is conserved in fruit fly and zebrafish. Both adopt familiar curved arrangements of HEAT-like repeats that bind 5' TOP mRNAs on the same conserved surface, although molecular dynamics simulations suggest that the N-terminal fold of the fruit fly DM15 is predicted to be unstable and unfold. We demonstrate that each ortholog interacts with TOP sequences with varying affinities. Importantly, we determine that the ability of the DM15 region to bind some TOP sequences but not others might amount to the context of the RNA structure, rather than the ability of the module to recognize some sequences but not others. We propose that TOP mRNAs may retain similar secondary structures to regulate LARP1 DM15 recognition.

PubMed: 39190712
DOI: 10.1371/journal.pone.0308574

実験手法

X-RAY DIFFRACTION (2.295 Å)