8DHT

Crystal structure of a typeIII Rubisco

8DHT の概要

• エントリーDOI: 10.2210/pdb8dht/pdb
• 分子名称: Ribulose bisphosphate carboxylase, MAGNESIUM ION, 3-PHOSPHOGLYCERIC ACID, ... (6 entities in total)
• 機能のキーワード: rubisco, 3-phosphoglycerate, complex, photosynthesis, lyase
• 由来する生物種: Archaeoglobus fulgidus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 197214.94

構造登録者

Qingqiu, H. (登録日: 2022-06-28, 公開日: 2022-11-30, 最終更新日: 2023-11-15)

主引用文献

Huang, Q.,Szebenyi, D.M.E.
Crystal structure of a type III Rubisco in complex with its product 3-phosphoglycerate.
Proteins, 91:330-337, 2023

PubMed Abstract: The crystal structure of the complex of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) from Archaeoglobus fulgidus (afRubisco) with its products 3PGAs has been determined to a resolution of 1.7 Å and is of the closed form. Type III Rubiscos such as afRubisco have 18 out of the 19 essential amino acid residues of canonical Rubisco; the 19th is Tyr rather than Phe. Superposition with the structure of a complex of the similar tkRubisco with the six-carbon intermediate analog 2CABP shows the same conformation of the 19 residues except for Glu46 and Thr51. Glu46 adopts a unique conformation different from that in other Rubiscos and makes two H-bonds with the ligand 3PGA. Similar to other closed state Rubiscos, the backbone of Thr51 is rotated and the side chain makes an H-bond with the ligand 3PGA. Two product 3PGA molecules are bound at the active site, overlapping well with the 2CABP of tkRubisco/2CABP. The positions of the P1 and P2 phosphate groups differ by 0.4 and 0.53 Å, respectively, between 2CABP and the two 3PGAs. This afRubisco/3PGA complex mimics an intermediate stage of the carboxylation reaction which occurs after the production of the two 3PGA products but before the reopening of the active site. The stability of this complex suggests that the Rubisco active site will not reopen before both 3PGA products are formed.

PubMed: 36151846
DOI: 10.1002/prot.26431

実験手法

X-RAY DIFFRACTION (1.699 Å)