8DGW

Crystal structure of HCoV-HKU1 spike stem helix peptide in complex with Fab of broadly neutralizing antibody CC95.108 isolated from a vaccinated COVID-19 convalescent

8DGW の概要

• エントリーDOI: 10.2210/pdb8dgw/pdb
• 分子名称: Antibody CC95.108 Fab heavy chain, Antibody CC95.108 Fab light chain, Spike protein S2' (3 entities in total)
• 機能のキーワード: broadly, neutralizing, antibody, pan-betacoronavirus, s2 stem helix, spike, sars-cov-2, mers-cov, hcov-hku1, sarbecovirus, cross-reactive, cross-neutralizing, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 196935.22

構造登録者

Liu, H.,Wilson, I.A. (登録日: 2022-06-24, 公開日: 2023-01-25, 最終更新日: 2024-10-30)

主引用文献

Zhou, P.,Song, G.,Liu, H.,Yuan, M.,He, W.T.,Beutler, N.,Zhu, X.,Tse, L.V.,Martinez, D.R.,Schafer, A.,Anzanello, F.,Yong, P.,Peng, L.,Dueker, K.,Musharrafieh, R.,Callaghan, S.,Capozzola, T.,Limbo, O.,Parren, M.,Garcia, E.,Rawlings, S.A.,Smith, D.M.,Nemazee, D.,Jardine, J.G.,Safonova, Y.,Briney, B.,Rogers, T.F.,Wilson, I.A.,Baric, R.S.,Gralinski, L.E.,Burton, D.R.,Andrabi, R.
Broadly neutralizing anti-S2 antibodies protect against all three human betacoronaviruses that cause deadly disease.
Immunity, 56:669-686.e7, 2023

PubMed Abstract: Pan-betacoronavirus neutralizing antibodies may hold the key to developing broadly protective vaccines against novel pandemic coronaviruses and to more effectively respond to SARS-CoV-2 variants. The emergence of Omicron and subvariants of SARS-CoV-2 illustrates the limitations of solely targeting the receptor-binding domain (RBD) of the spike (S) protein. Here, we isolated a large panel of broadly neutralizing antibodies (bnAbs) from SARS-CoV-2 recovered-vaccinated donors, which targets a conserved S2 region in the betacoronavirus spike fusion machinery. Select bnAbs showed broad in vivo protection against all three deadly betacoronaviruses, SARS-CoV-1, SARS-CoV-2, and MERS-CoV, which have spilled over into humans in the past two decades. Structural studies of these bnAbs delineated the molecular basis for their broad reactivity and revealed common antibody features targetable by broad vaccination strategies. These bnAbs provide new insights and opportunities for antibody-based interventions and for developing pan-betacoronavirus vaccines.

PubMed: 36889306
DOI: 10.1016/j.immuni.2023.02.005

実験手法

X-RAY DIFFRACTION (2.81 Å)