8DGO

Growth Factor Receptor-Bound Protein 2 (Grb2) bound to phosphorylated PEAK3 (pY24) peptide

Summary for 8DGO

• Entry DOI: 10.2210/pdb8dgo/pdb
• Descriptor: Growth factor receptor bound protein 2, Phosphorylated PEAK3 (pY24) peptide (3 entities in total)
• Functional Keywords: sh2 domain, signaling protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 3
• Total formula weight: 51672.77

Authors

Roy, M.J.,Hardy, J.M.,Lucet, I.S. (deposition date: 2022-06-24, release date: 2023-06-07, Last modification date: 2023-11-15)

Primary citation

Roy, M.J.,Surudoi, M.G.,Kropp, A.,Hou, J.,Dai, W.,Hardy, J.M.,Liang, L.Y.,Cotton, T.R.,Lechtenberg, B.C.,Dite, T.A.,Ma, X.,Daly, R.J.,Patel, O.,Lucet, I.S.
Structural mapping of PEAK pseudokinase interactions identifies 14-3-3 as a molecular switch for PEAK3 signaling.
Nat Commun, 14:3542-3542, 2023

PubMed Abstract: PEAK pseudokinases regulate cell migration, invasion and proliferation by recruiting key signaling proteins to the cytoskeleton. Despite lacking catalytic activity, alteration in their expression level is associated with several aggressive cancers. Here, we elucidate the molecular details of key PEAK signaling interactions with the adapter proteins CrkII and Grb2 and the scaffold protein 14-3-3. Our findings rationalize why the dimerization of PEAK proteins has a crucial function in signal transduction and provide biophysical and structural data to unravel binding specificity within the PEAK interactome. We identify a conserved high affinity 14-3-3 motif on PEAK3 and demonstrate its role as a molecular switch to regulate CrkII binding and signaling via Grb2. Together, our studies provide a detailed structural snapshot of PEAK interaction networks and further elucidate how PEAK proteins, especially PEAK3, act as dynamic scaffolds that exploit adapter proteins to control signal transduction in cell growth/motility and cancer.

PubMed: 37336884
DOI: 10.1038/s41467-023-38869-9

Experimental method

X-RAY DIFFRACTION (2.3 Å)