8DGL

Crystal Structure of the RdfS Excisionase

8DGL の概要

• エントリーDOI: 10.2210/pdb8dgl/pdb
• 分子名称: Recombination Directionality Factor RdfS, CHLORIDE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: excisionase, recombination directionality factor, winged helix-turn-helix, superhelix, dna binding protein
• 由来する生物種: Mesorhizobium japonicum R7A
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 40261.70

構造登録者

Verdonk, C.J.,Ramsay, J.P.,Marshall, A.C.,Bond, C.S. (登録日: 2022-06-24, 公開日: 2022-10-05, 最終更新日: 2024-04-03)

主引用文献

Verdonk, C.J.,Marshall, A.C.,Ramsay, J.P.,Bond, C.S.
Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element.
Acta Crystallogr D Struct Biol, 78:1210-1220, 2022

PubMed Abstract: The recombination directionality factors from Mesorhizobium spp. (RdfS) are involved in regulating the excision and transfer of integrative and conjugative elements. Here, solution small-angle X-ray scattering, and crystallization and preliminary structure solution of RdfS from Mesorhizobium japonicum R7A are presented. RdfS crystallizes in space group P222, with evidence of eightfold rotational crystallographic/noncrystallographic symmetry. Initial structure determination by molecular replacement using ab initio models yielded a partial model (three molecules), which was completed after manual inspection revealed unmodelled electron density. The finalized crystal structure of RdfS reveals a head-to-tail polymer forming left-handed superhelices with large solvent channels. Additionally, RdfS has significant disorder in the C-terminal region of the protein, which is supported by the solution scattering data and the crystal structure. The steps taken to finalize structure determination, as well as the scattering and crystallographic characteristics of RdfS, are discussed.

PubMed: 36189741
DOI: 10.1107/S2059798322008579

実験手法

X-RAY DIFFRACTION (2.45 Å)