8DFT

Cryo-EM structure of conjugative pili from Pyrobaculum calidifontis

8DFT の概要

• エントリーDOI: 10.2210/pdb8dft/pdb
• EMDBエントリー: 27413
• 分子名称: Pilin protein, [(2~{S},7~{S},11~{S},15~{S},19~{R},22~{R},26~{S},30~{R},34~{R},38~{S},43~{S},47~{S},51~{S},55~{R},58~{R},62~{S},66~{R},70~{R})-38-(hydroxymethyl)-7,11,15,19,22,26,30,34,43,47,51,55,58,62,66,70-hexadecamethyl-1,4,37,40-tetraoxacyclodoheptacont-2-yl]methanol (2 entities in total)
• 機能のキーワード: archaeal conjugative pilus, protein fibril
• 由来する生物種: Pyrobaculum calidifontis
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 264907.78

構造登録者

Beltran, L.C.,Egelman, E.H. (登録日: 2022-06-22, 公開日: 2023-03-22, 最終更新日: 2024-09-25)

主引用文献

Beltran, L.C.,Cvirkaite-Krupovic, V.,Miller, J.,Wang, F.,Kreutzberger, M.A.B.,Patkowski, J.B.,Costa, T.R.D.,Schouten, S.,Levental, I.,Conticello, V.P.,Egelman, E.H.,Krupovic, M.
Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery.
Nat Commun, 14:666-666, 2023

PubMed Abstract: Conjugation is a major mechanism of horizontal gene transfer promoting the spread of antibiotic resistance among human pathogens. It involves establishing a junction between a donor and a recipient cell via an extracellular appendage known as the mating pilus. In bacteria, the conjugation machinery is encoded by plasmids or transposons and typically mediates the transfer of cognate mobile genetic elements. Much less is known about conjugation in archaea. Here, we determine atomic structures by cryo-electron microscopy of three conjugative pili, two from hyperthermophilic archaea (Aeropyrum pernix and Pyrobaculum calidifontis) and one encoded by the Ti plasmid of the bacterium Agrobacterium tumefaciens, and show that the archaeal pili are homologous to bacterial mating pili. However, the archaeal conjugation machinery, known as Ced, has been 'domesticated', that is, the genes for the conjugation machinery are encoded on the chromosome rather than on mobile genetic elements, and mediates the transfer of cellular DNA.

PubMed: 36750723
DOI: 10.1038/s41467-023-36349-8

実験手法

ELECTRON MICROSCOPY (4.1 Å)