8DF8

Structure of M. kandleri topoisomerase V in complex with DNA. 40 base pair symmetric DNA complex

8DF8 の概要

• エントリーDOI: 10.2210/pdb8df8/pdb
• 分子名称: Topoisomerase V, DNA (42-MER), POTASSIUM ION, ... (6 entities in total)
• 機能のキーワード: topoisomerase v type ic, abasic dna, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Methanopyrus kandleri; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 220776.73

構造登録者

Osterman, A.,Mondragon, A. (登録日: 2022-06-21, 公開日: 2022-08-31, 最終更新日: 2023-10-18)

主引用文献

Osterman, A.,Mondragon, A.
Structures of topoisomerase V in complex with DNA reveal unusual DNA binding mode and novel relaxation mechanism.
Elife, 11:-, 2022

PubMed Abstract: Topoisomerase V is a unique topoisomerase that combines DNA repair and topoisomerase activities. The enzyme has an unusual arrangement, with a small topoisomerase domain followed by 12 tandem (HhH) domains, which include 3 AP lyase repair domains. The uncommon architecture of this enzyme bears no resemblance to any other known topoisomerase. Here, we present structures of topoisomerase V in complex with DNA. The structures show that the (HhH) domains wrap around the DNA and in this manner appear to act as a processivity factor. There is a conformational change in the protein to expose the topoisomerase active site. The DNA bends sharply to enter the active site, which melts the DNA and probably facilitates relaxation. The structures show a DNA-binding mode not observed before and provide information on the way this atypical topoisomerase relaxes DNA. In common with type IB enzymes, topoisomerase V relaxes DNA using a controlled rotation mechanism, but the structures show that topoisomerase V accomplishes this in different manner. Overall, the structures firmly establish that type IC topoisomerases form a distinct type of topoisomerases, with no similarities to other types at the sequence, structural, or mechanistic level. They represent a completely different solution to DNA relaxation.

PubMed: 35969036
DOI: 10.7554/eLife.72702

実験手法

X-RAY DIFFRACTION (2.92 Å)