8D9S

AP-1, Arf1, Nef lattice on MHC-I lipopeptide incorporated wide membrane tubes, centered on beta-Arf1

8D9S の概要

• エントリーDOI: 10.2210/pdb8d9s/pdb
• EMDBエントリー: 27183 27190
• 分子名称: ADP ribosylation factor 1, Protein Nef, HLA class I histocompatibility antigen, A alpha chain, ... (9 entities in total)
• 機能のキーワード: trafficking, ap, hiv, viral protein-protein transport complex, viral protein/protein transport
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 2142956.47

構造登録者

Hooy, R.M.,Hurley, J.H. (登録日: 2022-06-11, 公開日: 2022-11-09, 最終更新日: 2024-06-12)

主引用文献

Hooy, R.M.,Iwamoto, Y.,Tudorica, D.A.,Ren, X.,Hurley, J.H.
Self-assembly and structure of a clathrin-independent AP-1:Arf1 tubular membrane coat.
Sci Adv, 8:eadd3914-eadd3914, 2022

PubMed Abstract: The adaptor protein (AP) complexes not only form the inner layer of clathrin coats but also have clathrin-independent roles in membrane traffic whose mechanisms are unknown. HIV-1 Nef hijacks AP-1 to sequester major histocompatibility complex class I (MHC-I), evading immune detection. We found that AP-1:Arf1:Nef:MHC-I forms a coat on tubulated membranes without clathrin and determined its structure. The coat assembles via Arf1 dimer interfaces. AP-1-positive tubules are enriched in cells upon clathrin knockdown. Nef localizes preferentially to AP-1 tubules in cells, explaining how Nef sequesters MHC-I. Coat contact residues are conserved across Arf isoforms and the Arf-dependent AP complexes AP-1, AP-3, and AP-4. Thus, AP complexes can self-assemble with Arf1 into tubular coats without clathrin or other scaffolding factors. The AP-1:Arf1 coat defines the structural basis of a broader class of tubulovesicular membrane coats as an intermediate in clathrin vesicle formation from internal membranes and as an MHC-I sequestration mechanism in HIV-1 infection.

PubMed: 36269825
DOI: 10.1126/sciadv.add3914

実験手法

ELECTRON MICROSCOPY (20 Å)