8D71

Human Ago2 bound to miR122(21nt)

8D71 の概要

• エントリーDOI: 10.2210/pdb8d71/pdb
• 分子名称: Protein argonaute-2, miR-122-21nt, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: argonaute, rnai, microrna, mirna, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104214.52

構造登録者

Xiao, Y.,MacRae, I. (登録日: 2022-06-07, 公開日: 2023-05-03, 最終更新日: 2023-10-25)

主引用文献

Xiao, Y.,Liu, T.M.,MacRae, I.J.
A tiny loop in the Argonaute PIWI domain tunes small RNA seed strength.
Embo Rep., 24:e55806-e55806, 2023

PubMed Abstract: Argonaute (AGO) proteins use microRNAs (miRNAs) and small interfering RNAs (siRNAs) as guides to regulate gene expression in plants and animals. AGOs that use miRNAs in bilaterian animals recognize short (6-8 nt.) elements complementary to the miRNA seed region, enabling each miRNA to interact with hundreds of otherwise unrelated targets. By contrast, AGOs that use miRNAs in plants employ longer (> 13 nt.) recognition elements such that each miRNA silences a small number of physiologically related targets. Here, we show that this major functional distinction depends on a minor structural difference between plant and animal AGO proteins: a 9-amino acid loop in the PIWI domain. Swapping the PIWI loop from human Argonaute2 (HsAGO2) into Arabidopsis Argonaute10 (AtAGO10) increases seed strength, resulting in animal-like miRNA targeting. Conversely, swapping the plant PIWI loop into HsAGO2 reduces seed strength and accelerates the turnover of cleaved targets. The loop-swapped HsAGO2 silences targets more potently, with reduced miRNA-like targeting, than wild-type HsAGO2 in mammalian cells. Thus, tiny structural differences can tune the targeting properties of AGO proteins for distinct biological roles.

PubMed: 37082939
DOI: 10.15252/embr.202255806

実験手法

X-RAY DIFFRACTION (2.5 Å)