8D64

ELIC with cysteamine in POPC nanodisc

8D64 の概要

• エントリーDOI: 10.2210/pdb8d64/pdb
• 関連するPDBエントリー: 2YN6 6V03 8D63 8D65 8D66 8D67 8D68
• EMDBエントリー: 27215 27216 27217 27218 27219 27220
• 分子名称: Erwinia ligand-gated ion channel, 2-AMINO-ETHANETHIOL (2 entities in total)
• 機能のキーワード: elic, ion channel, plgic, structural protein
• 由来する生物種: Dickeya dadantii
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 177302.58

構造登録者

Petroff II, J.T.,Deng, Z.,Rau, M.J.,Fitzpatrick, J.A.J.,Yuan, P.,Cheng, W.W.L. (登録日: 2022-06-06, 公開日: 2022-11-23, 最終更新日: 2024-06-12)

主引用文献

Petroff II, J.T.,Dietzen, N.M.,Santiago-McRae, E.,Deng, B.,Washington, M.S.,Chen, L.J.,Trent Moreland, K.,Deng, Z.,Rau, M.,Fitzpatrick, J.A.J.,Yuan, P.,Joseph, T.T.,Henin, J.,Brannigan, G.,Cheng, W.W.L.
Open-channel structure of a pentameric ligand-gated ion channel reveals a mechanism of leaflet-specific phospholipid modulation.
Nat Commun, 13:7017-7017, 2022

PubMed Abstract: Pentameric ligand-gated ion channels (pLGICs) mediate synaptic transmission and are sensitive to their lipid environment. The mechanism of phospholipid modulation of any pLGIC is not well understood. We demonstrate that the model pLGIC, ELIC (Erwinia ligand-gated ion channel), is positively modulated by the anionic phospholipid, phosphatidylglycerol, from the outer leaflet of the membrane. To explore the mechanism of phosphatidylglycerol modulation, we determine a structure of ELIC in an open-channel conformation. The structure shows a bound phospholipid in an outer leaflet site, and structural changes in the phospholipid binding site unique to the open-channel. In combination with streamlined alchemical free energy perturbation calculations and functional measurements in asymmetric liposomes, the data support a mechanism by which an anionic phospholipid stabilizes the activated, open-channel state of a pLGIC by specific, state-dependent binding to this site.

PubMed: 36385237
DOI: 10.1038/s41467-022-34813-5

実験手法

ELECTRON MICROSCOPY (3.14 Å)