8D3Z

Crystal structure of GalS1 in complex with Manganese from Populus trichocarpas

8D3Z の概要

• エントリーDOI: 10.2210/pdb8d3z/pdb
• 分子名称: Galactan synthase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: glycosyltransferase galactan synthase, transferase
• 由来する生物種: Populus trichocarpa (Western balsam poplar, Populus balsamifera subsp. trichocarpa)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 102889.13

構造登録者

Pereira, J.H.,Prabhakar, P.K.,Urbanowicz, B.R.,Adams, P.D. (登録日: 2022-06-01, 公開日: 2023-03-15, 最終更新日: 2024-10-30)

主引用文献

Prabhakar, P.K.,Pereira, J.H.,Taujale, R.,Shao, W.,Bharadwaj, V.S.,Chapla, D.,Yang, J.Y.,Bomble, Y.J.,Moremen, K.W.,Kannan, N.,Hammel, M.,Adams, P.D.,Scheller, H.V.,Urbanowicz, B.R.
Structural and biochemical insight into a modular beta-1,4-galactan synthase in plants.
Nat.Plants, 9:486-500, 2023

PubMed Abstract: Rhamnogalacturonan I (RGI) is a structurally complex pectic polysaccharide with a backbone of alternating rhamnose and galacturonic acid residues substituted with arabinan and galactan side chains. Galactan synthase 1 (GalS1) transfers galactose and arabinose to either extend or cap the β-1,4-galactan side chains of RGI, respectively. Here we report the structure of GalS1 from Populus trichocarpa, showing a modular protein consisting of an N-terminal domain that represents the founding member of a new family of carbohydrate-binding module, CBM95, and a C-terminal glycosyltransferase family 92 (GT92) catalytic domain that adopts a GT-A fold. GalS1 exists as a dimer in vitro, with stem domains interacting across the chains in a 'handshake' orientation that is essential for maintaining stability and activity. In addition to understanding the enzymatic mechanism of GalS1, we gained insight into the donor and acceptor substrate binding sites using deep evolutionary analysis, molecular simulations and biochemical studies. Combining all the results, a mechanism for GalS1 catalysis and a new model for pectic galactan side-chain addition are proposed.

PubMed: 36849618
DOI: 10.1038/s41477-023-01358-4

実験手法

X-RAY DIFFRACTION (2.56 Å)