8D2J

A novel insecticidal protein from ferns IPD113_Cow.

8D2J の概要

• エントリーDOI: 10.2210/pdb8d2j/pdb
• 分子名称: IPD113_Cow, GLYCEROL (3 entities in total)
• 機能のキーワード: insecticidal protein, plant protein
• 由来する生物種: Colysis wrightii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 233266.00

構造登録者

Maher, M.J. (登録日: 2022-05-30, 公開日: 2023-11-08, 最終更新日: 2024-10-16)

主引用文献

Wei, J.Z.,Lum, A.,Schepers, E.,Liu, L.,Weston, R.T.,McGinness, B.S.,Heckert, M.J.,Xie, W.,Kassa, A.,Bruck, D.,Rauscher, G.,Kapka-Kitzman, D.,Mathis, J.P.,Zhao, J.Z.,Sethi, A.,Barry, J.,Lu, A.L.,Brugliera, F.,Lee, E.L.,van derWeerden, N.L.,Eswar, N.,Maher, M.J.,Anderson, M.A.
Novel insecticidal proteins from ferns resemble insecticidal proteins from Bacillus thuringiensis.
Proc.Natl.Acad.Sci.USA, 120:e2306177120-e2306177120, 2023

PubMed Abstract: Lepidopterans affect crop production worldwide. The use of transgenes encoding insecticidal proteins from () in crop plants is a well-established technology that enhances protection against lepidopteran larvae. Concern about widespread field-evolved resistance to proteins has highlighted an urgent need for new insecticidal proteins with different modes or sites of action. We discovered a new family of insecticidal proteins from ferns. The prototype protein from species (Order Polypodiales) and variants from two other orders of ferns, Schizaeales and Ophioglossales, were effective against important lepidopteran pests of maize and soybean in diet-based assays. Transgenic maize and soybean plants producing these proteins were more resistant to insect damage than controls. We report here the crystal structure of a variant of the prototype protein to 1.98 Å resolution. Remarkably, despite being derived from plants, the structure resembles the 3-domain Cry proteins from but has only two out of three of their characteristic domains, lacking the C-terminal domain which is typically required for their activities. Two of the fern proteins were effective against strains of fall armyworm that were resistant to 3-domain Cry proteins Cry1Fa or Cry2A.127. This therefore represents a novel family of insecticidal proteins that have the potential to provide future tools for pest control.

PubMed: 37871210
DOI: 10.1073/pnas.2306177120

実験手法

X-RAY DIFFRACTION (2.1 Å)