8CYE

Cryo-EM asymmetric reconstruction of the EPEC H6 bacterial flagellar filament Normal Waveform

8CYE の概要

• エントリーDOI: 10.2210/pdb8cye/pdb
• EMDBエントリー: 27059 27060 27064 27065 27076
• 分子名称: Flagellin (1 entity in total)
• 機能のキーワード: bacterial flagellum, flagellin, bacterial motility, supercoiling, structural protein
• 由来する生物種: Escherichia coli O127:H6
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 1239524.18

構造登録者

Kreutzberger, M.A.B.,Chatterjee, S.,Frankel, G.,Egelman, E.H. (登録日: 2022-05-23, 公開日: 2022-09-07, 最終更新日: 2025-06-04)

主引用文献

Kreutzberger, M.A.B.,Sonani, R.R.,Liu, J.,Chatterjee, S.,Wang, F.,Sebastian, A.L.,Biswas, P.,Ewing, C.,Zheng, W.,Poly, F.,Frankel, G.,Luisi, B.F.,Calladine, C.R.,Krupovic, M.,Scharf, B.E.,Egelman, E.H.
Convergent evolution in the supercoiling of prokaryotic flagellar filaments.
Cell, 185:3487-3500.e14, 2022

PubMed Abstract: The supercoiling of bacterial and archaeal flagellar filaments is required for motility. Archaeal flagellar filaments have no homology to their bacterial counterparts and are instead homologs of bacterial type IV pili. How these prokaryotic flagellar filaments, each composed of thousands of copies of identical subunits, can form stable supercoils under torsional stress is a fascinating puzzle for which structural insights have been elusive. Advances in cryoelectron microscopy (cryo-EM) make it now possible to directly visualize the basis for supercoiling, and here, we show the atomic structures of supercoiled bacterial and archaeal flagellar filaments. For the bacterial flagellar filament, we identify 11 distinct protofilament conformations with three broad classes of inter-protomer interface. For the archaeal flagellar filament, 10 protofilaments form a supercoil geometry supported by 10 distinct conformations, with one inter-protomer discontinuity creating a seam inside of the curve. Our results suggest that convergent evolution has yielded stable superhelical geometries that enable microbial locomotion.

PubMed: 36057255
DOI: 10.1016/j.cell.2022.08.009

実験手法

ELECTRON MICROSCOPY (3.9 Å)