8CVW

20ns Temperature-Jump (Dark2) XFEL structure of Lysozyme

8CVW の概要

• エントリーDOI: 10.2210/pdb8cvw/pdb
• 分子名称: Lysozyme C, SODIUM ION, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: lysozyme, temperature-jump, dark2, 20ns, xfel, hydrolase
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14649.50

構造登録者

Wolff, A.M.,Thompson, M.C.,Fraser, J.S.,Nango, E. (登録日: 2022-05-18, 公開日: 2022-06-22, 最終更新日: 2024-10-16)

主引用文献

Wolff, A.M.,Nango, E.,Young, I.D.,Brewster, A.S.,Kubo, M.,Nomura, T.,Sugahara, M.,Owada, S.,Barad, B.A.,Ito, K.,Bhowmick, A.,Carbajo, S.,Hino, T.,Holton, J.M.,Im, D.,O'Riordan, L.J.,Tanaka, T.,Tanaka, R.,Sierra, R.G.,Yumoto, F.,Tono, K.,Iwata, S.,Sauter, N.K.,Fraser, J.S.,Thompson, M.C.
Mapping protein dynamics at high spatial resolution with temperature-jump X-ray crystallography.
Nat.Chem., 15:1549-1558, 2023

PubMed Abstract: Understanding and controlling protein motion at atomic resolution is a hallmark challenge for structural biologists and protein engineers because conformational dynamics are essential for complex functions such as enzyme catalysis and allosteric regulation. Time-resolved crystallography offers a window into protein motions, yet without a universal perturbation to initiate conformational changes the method has been limited in scope. Here we couple a solvent-based temperature jump with time-resolved crystallography to visualize structural motions in lysozyme, a dynamic enzyme. We observed widespread atomic vibrations on the nanosecond timescale, which evolve on the submillisecond timescale into localized structural fluctuations that are coupled to the active site. An orthogonal perturbation to the enzyme, inhibitor binding, altered these dynamics by blocking key motions that allow energy to dissipate from vibrations into functional movements linked to the catalytic cycle. Because temperature jump is a universal method for perturbing molecular motion, the method demonstrated here is broadly applicable for studying protein dynamics.

PubMed: 37723259
DOI: 10.1038/s41557-023-01329-4

実験手法

X-RAY DIFFRACTION (1.57 Å)