8CSS

Human mitochondrial small subunit assembly intermediate (State D)

8CSS の概要

• エントリーDOI: 10.2210/pdb8css/pdb
• EMDBエントリー: 26969
• 分子名称: 28S ribosomal protein S34, mitochondrial, 28S ribosomal protein S24, mitochondrial, 28S ribosomal protein S5, mitochondrial, ... (43 entities in total)
• 機能のキーワード: ribonucleoprotein complex mitochondria biogenesis, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 33
• 化学式量合計: 1264763.99

構造登録者

Harper, N.J.,Burnside, C.,Klinge, S. (登録日: 2022-05-13, 公開日: 2022-12-14, 最終更新日: 2023-02-08)

主引用文献

Harper, N.J.,Burnside, C.,Klinge, S.
Principles of mitoribosomal small subunit assembly in eukaryotes.
Nature, 614:175-181, 2023

PubMed Abstract: Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies.

PubMed: 36482135
DOI: 10.1038/s41586-022-05621-0

実験手法

ELECTRON MICROSCOPY (2.36 Å)