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8CSH

Structure of the DNA binding domain of pSK1 Par partition protein bound to centromere DNA

8CSH の概要
エントリーDOI10.2210/pdb8csh/pdb
分子名称DNA (5'-D(P*TP*GP*TP*TP*AP*GP*GP*TP*AP*CP*CP*TP*AP*AP*C)-3'), Par, DNA (5'-D(*TP*AP*GP*TP*TP*AP*GP*GP*TP*AP*CP*CP*TP*AP*AP*CP*A)-3'), ... (4 entities in total)
機能のキーワードdna segregation, psk1, par protein, centromere dna, dna binding protein-dna complex, dna binding protein/dna
由来する生物種unidentified plasmid
詳細
タンパク質・核酸の鎖数4
化学式量合計50051.17
構造登録者
Schumacher, M.A. (登録日: 2022-05-12, 公開日: 2022-08-10, 最終更新日: 2024-02-14)
主引用文献Chan, H.Y.,Jensen, S.O.,LeBard, R.J.,Figgett, W.A.,Lai, E.,Simpson, A.E.,Brzoska, A.J.,Davies, D.S.,Connolly, A.M.,Cordwell, S.J.,Travis, B.A.,Salinas, R.,Skurray, R.A.,Firth, N.,Schumacher, M.A.
Molecular Analysis of pSK1 par: A Novel Plasmid Partitioning System Encoded by Staphylococcal Multiresistance Plasmids.
J.Mol.Biol., 434:167770-167770, 2022
Cited by
PubMed Abstract: The segregation of prokaryotic plasmids typically requires a centromere-like site and two proteins, a centromere-binding protein (CBP) and an NTPase. By contrast, a single 245 residue Par protein mediates partition of the prototypical staphylococcal multiresistance plasmid pSK1 in the absence of an identifiable NTPase component. To gain insight into centromere binding by pSK1 Par and its segregation function we performed structural, biochemical and in vivo studies. Here we show that pSK1 Par binds a centromere consisting of seven repeat elements. We demonstrate this Par-centromere interaction also mediates Par autoregulation. To elucidate the Par centromere binding mechanism, we obtained a structure of the Par N-terminal DNA-binding domain bound to centromere DNA to 2.25 Å. The pSK1 Par structure, which harbors a winged-helix-turn-helix (wHTH), is distinct from other plasmid CBP structures but shows homology to the B. subtilis chromosome segregation protein, RacA. Biochemical studies suggest the region C-terminal to the Par wHTH forms coiled coils and mediates oligomerization. Fluorescence microscopy analyses show that pSK1 Par enhances the separation of plasmids from clusters, driving effective segregation upon cell division. Combined the data provide insight into the molecular properties of a single protein partition system.
PubMed: 35907571
DOI: 10.1016/j.jmb.2022.167770
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.25 Å)
構造検証レポート
Validation report summary of 8csh
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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