8CRA

Structure of the keratin-like domain of SEPALLATA3 and AGAMOUS from Arabidopsis thaliana

Summary for 8CRA

• Entry DOI: 10.2210/pdb8cra/pdb
• Descriptor: Floral homeotic protein AGAMOUS, Developmental protein SEPALLATA 3 (3 entities in total)
• Functional Keywords: mads transcription factor, floral organ development, carpel development, tetramerization domain, heterotetramer, plant protein
• Biological source: Arabidopsis thaliana (thale cress); More
• Total number of polymer chains: 8
• Total formula weight: 91007.04

Authors

Zubieta, C.,Hugouvieux, V. (deposition date: 2023-03-08, release date: 2024-03-27, Last modification date: 2024-10-09)

Primary citation

Hugouvieux, V.,Blanc-Mathieu, R.,Janeau, A.,Paul, M.,Lucas, J.,Xu, X.,Ye, H.,Lai, X.,Le Hir, S.,Guillotin, A.,Galien, A.,Yan, W.,Nanao, M.,Kaufmann, K.,Parcy, F.,Zubieta, C.
SEPALLATA-driven MADS transcription factor tetramerization is required for inner whorl floral organ development.
Plant Cell, 36:3435-3450, 2024

PubMed Abstract: MADS transcription factors are master regulators of plant reproduction and flower development. The SEPALLATA (SEP) subfamily of MADS transcription factors is required for the development of floral organs and plays roles in inflorescence architecture and development of the floral meristem. SEPALLATAs act as organizers of MADS complexes, forming both heterodimers and heterotetramers in vitro. To date, the MADS complexes characterized in angiosperm floral organ development contain at least 1 SEPALLATA protein. Whether DNA binding by SEPALLATA-containing dimeric MADS complexes is sufficient for launching floral organ identity programs, however, is not clear as only defects in floral meristem determinacy were observed in tetramerization-impaired SEPALLATA mutant proteins. Here, we used a combination of genome-wide-binding studies, high-resolution structural studies of the SEP3/AGAMOUS (AG) tetramerization domain, structure-based mutagenesis and complementation experiments in Arabidopsis (Arabidopsis thaliana) sep1 sep2 sep3 and sep1 sep2 sep3 ag-4 plants transformed with versions of SEP3 encoding tetramerization mutants. We demonstrate that while SEP3 heterodimers can bind DNA both in vitro and in vivo and recognize the majority of SEP3 wild-type-binding sites genome-wide, tetramerization is required not only for floral meristem determinacy but also for floral organ identity in the second, third, and fourth whorls.

PubMed: 38771250
DOI: 10.1093/plcell/koae151

Experimental method

X-RAY DIFFRACTION (2.4 Å)