8CMR

Linear specific OTU-type DUB SnOTU from the pathogen S. negenvensis in complex with linear di-ubiquitin

8CMR の概要

• エントリーDOI: 10.2210/pdb8cmr/pdb
• 分子名称: OTU domain-containing protein, Polyubiquitin-B (3 entities in total)
• 機能のキーワード: deubiquitinase, papain-fold, otu, hydrolase, swapped complex
• 由来する生物種: Simkania negevensis Z; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 102029.17

構造登録者

Uthoff, M.,Hermanns, T.,Boll, V.,Hofmann, K.,Baumann, U. (登録日: 2023-02-21, 公開日: 2023-11-22)

主引用文献

Boll, V.,Hermanns, T.,Uthoff, M.,Erven, I.,Horner, E.M.,Kozjak-Pavlovic, V.,Baumann, U.,Hofmann, K.
Functional and structural diversity in deubiquitinases of the Chlamydia-like bacterium Simkania negevensis.
Nat Commun, 14:7335-7335, 2023

PubMed Abstract: Besides the regulation of many cellular pathways, ubiquitination is important for defense against invading pathogens. Some intracellular bacteria have evolved deubiquitinase (DUB) effector proteins, which interfere with the host ubiquitin system and help the pathogen to evade xenophagy and lysosomal degradation. Most intracellular bacteria encode one or two DUBs, which are often linkage-promiscuous or preferentially cleave K63-linked chains attached to bacteria or bacteria-containing vacuoles. By contrast, the respiratory pathogen Legionella pneumophila possesses a much larger number of DUB effectors, including a K6-specific enzyme belonging to the OTU family and an M1-specific DUB uniquely found in this bacterium. Here, we report that the opportunistic pathogen Simkania negevensis, which is unrelated to Legionella but has a similar lifestyle, encodes a similarly large number of DUBs, including M1- and K6-specific enzymes. Simkania DUBs are highly diverse and include DUB classes never before seen in bacteria. Interestingly, the M1- and K6-specific DUBs of Legionella and Simkania are unrelated, suggesting that their acquisition occurred independently. We characterize the DUB activity of eight Simkania-encoded enzymes belonging to five different DUB classes. We also provide a structural basis for the M1-specificity of a Simkania DUB, which most likely evolved from a eukaryotic otubain-like precursor.

PubMed: 37957213
DOI: 10.1038/s41467-023-43144-y

実験手法

X-RAY DIFFRACTION (2.24 Å)