8CKA

Deinococcus radidurans HPI S-layer

8CKA の概要

• エントリーDOI: 10.2210/pdb8cka/pdb
• EMDBエントリー: 16694
• 分子名称: Hexagonally packed intermediate-layer surface protein (1 entity in total)
• 機能のキーワード: hpi s-layer, structural protein
• 由来する生物種: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 198848.00

構造登録者

von Kuegelgen, A.,Yamashita, K.,Murshudov, G.,Bharat, T. (登録日: 2023-02-14, 公開日: 2023-04-19, 最終更新日: 2024-10-23)

主引用文献

von Kugelgen, A.,van Dorst, S.,Yamashita, K.,Sexton, D.L.,Tocheva, E.I.,Murshudov, G.,Alva, V.,Bharat, T.A.M.
Interdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacterium Deinococcus radiodurans.
Proc.Natl.Acad.Sci.USA, 120:e2215808120-e2215808120, 2023

PubMed Abstract: is an atypical diderm bacterium with a remarkable ability to tolerate various environmental stresses, due in part to its complex cell envelope encapsulated within a hyperstable surface layer (S-layer). Despite decades of research on this cell envelope, atomic structural details of the S-layer have remained obscure. In this study, we report the electron cryomicroscopy structure of the S-layer, showing how it is formed by the Hexagonally Packed Intermediate-layer (HPI) protein arranged in a planar hexagonal lattice. The HPI protein forms an array of immunoglobulin-like folds within the S-layer, with each monomer extending into the adjacent hexamer, resulting in a highly interconnected, stable, sheet-like arrangement. Using electron cryotomography and subtomogram averaging from focused ion beam-milled cells, we have obtained a structure of the cellular S-layer, showing how this HPI S-layer coats native membranes on the surface of cells. Our S-layer structure from the diderm bacterium shows similarities to immunoglobulin-like domain-containing S-layers from monoderm bacteria and archaea, highlighting common features in cell surface organization across different domains of life, with connotations on the evolution of immunoglobulin-based molecular recognition systems in eukaryotes.

PubMed: 37043530
DOI: 10.1073/pnas.2215808120

実験手法

ELECTRON MICROSCOPY (2.52 Å)