8CJW
Nucleoprotein Thogotovirus delta188-196
Summary for 8CJW
| Entry DOI | 10.2210/pdb8cjw/pdb |
| Descriptor | Nucleoprotein, SUCCINIC ACID, 1,2-ETHANEDIOL, ... (5 entities in total) |
| Functional Keywords | viral replication, nucleoprotein, rna binding, orthomyxovirus, viral protein |
| Biological source | Thogotovirus thogotoense |
| Total number of polymer chains | 1 |
| Total formula weight | 50366.47 |
| Authors | |
| Primary citation | Dick, A.,Mikirtumov, V.,Fuchs, J.,Krupp, F.,Olal, D.,Bendl, E.,Sprink, T.,Diebolder, C.,Kudryashev, M.,Kochs, G.,Roske, Y.,Daumke, O. Structural characterization of Thogoto Virus nucleoprotein provides insights into viral RNA encapsidation and RNP assembly. Structure, 32:1068-1078.e5, 2024 Cited by PubMed Abstract: Orthomyxoviruses, such as influenza and thogotoviruses, are important human and animal pathogens. Their segmented viral RNA genomes are wrapped by viral nucleoproteins (NPs) into helical ribonucleoprotein complexes (RNPs). NP structures of several influenza viruses have been reported. However, there are still contradictory models of how orthomyxovirus RNPs are assembled. Here, we characterize the crystal structure of Thogoto virus (THOV) NP and found striking similarities to structures of influenza viral NPs, including a two-lobed domain architecture, a positively charged RNA-binding cleft, and a tail loop important for trimerization and viral transcription. A low-resolution cryo-electron tomography reconstruction of THOV RNPs elucidates a left-handed double helical assembly. By providing a model for RNP assembly of THOV, our study suggests conserved NP assembly and RNA encapsidation modes for thogoto- and influenza viruses. PubMed: 38749445DOI: 10.1016/j.str.2024.04.016 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.01 Å) |
Structure validation
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