8CJW

Nucleoprotein Thogotovirus delta188-196

8CJW の概要

• エントリーDOI: 10.2210/pdb8cjw/pdb
• 分子名称: Nucleoprotein, SUCCINIC ACID, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: viral replication, nucleoprotein, rna binding, orthomyxovirus, viral protein
• 由来する生物種: Thogotovirus thogotoense
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50366.47

構造登録者

Dick, A.,Roske, Y. (登録日: 2023-02-13, 公開日: 2024-06-19, 最終更新日: 2024-08-21)

主引用文献

Dick, A.,Mikirtumov, V.,Fuchs, J.,Krupp, F.,Olal, D.,Bendl, E.,Sprink, T.,Diebolder, C.,Kudryashev, M.,Kochs, G.,Roske, Y.,Daumke, O.
Structural characterization of Thogoto Virus nucleoprotein provides insights into viral RNA encapsidation and RNP assembly.
Structure, 32:1068-1078.e5, 2024

PubMed Abstract: Orthomyxoviruses, such as influenza and thogotoviruses, are important human and animal pathogens. Their segmented viral RNA genomes are wrapped by viral nucleoproteins (NPs) into helical ribonucleoprotein complexes (RNPs). NP structures of several influenza viruses have been reported. However, there are still contradictory models of how orthomyxovirus RNPs are assembled. Here, we characterize the crystal structure of Thogoto virus (THOV) NP and found striking similarities to structures of influenza viral NPs, including a two-lobed domain architecture, a positively charged RNA-binding cleft, and a tail loop important for trimerization and viral transcription. A low-resolution cryo-electron tomography reconstruction of THOV RNPs elucidates a left-handed double helical assembly. By providing a model for RNP assembly of THOV, our study suggests conserved NP assembly and RNA encapsidation modes for thogoto- and influenza viruses.

PubMed: 38749445
DOI: 10.1016/j.str.2024.04.016

実験手法

X-RAY DIFFRACTION (2.01 Å)