8CJ9

Crystal structure of maize CKO/CKX8 in complex with urea-derived inhibitor 2-[(3,5-dichlorophenyl)carbamoylamino]benzamide

8CJ9 の概要

• エントリーDOI: 10.2210/pdb8cj9/pdb
• 分子名称: cytokinin dehydrogenase, FLAVIN-ADENINE DINUCLEOTIDE, 2-[[3,5-bis(chloranyl)phenyl]carbamoylamino]benzamide, ... (6 entities in total)
• 機能のキーワード: hormone degradation, flavoprotein
• 由来する生物種: Zea mays
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 120211.23

構造登録者

Kopecny, D.,Briozzo, P.,Morera, S. (登録日: 2023-02-12, 公開日: 2024-02-21, 最終更新日: 2024-10-23)

主引用文献

Nisler, J.,Klimes, P.,Koncitikova, R.,Kadlecova, A.,Voller, J.,Chalaki, M.,Karampelias, M.,Murvanidze, N.,Werbrouck, S.P.O.,Kopecny, D.,Havlicek, L.,De Diego, N.,Briozzo, P.,Morera, S.,Zalabak, D.,Spichal, L.
Cytokinin oxidase/dehydrogenase inhibitors: progress towards agricultural practice.
J.Exp.Bot., 75:4873-4890, 2024

PubMed Abstract: Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve the efficiency of agriculture and plant tissue culture-based practices. Here, we report a synthesis and structure-activity relationship study of novel urea derivatives concerning their CKX inhibitory activity. The most active compounds showed sub-nanomolar IC50 values with maize ZmCKX1, the lowest value yet documented. Other CKX isoforms of maize and Arabidopsis were also inhibited very effectively. The binding mode of four compounds was characterized based on high-resolution crystal complex structures. Using the soil nematode Caenorhabditis elegans, and human skin fibroblasts, key CKX inhibitors with low toxicity were identified. These compounds enhanced the shoot regeneration of Lobelia, Drosera, and Plectranthus, as well as the growth of Arabidopsis and Brassica napus. At the same time, a key compound (identified as 82) activated a cytokinin primary response gene, ARR5:GUS, and a cytokinin sensor, TCSv2:GUS, without activating the Arabidopsis cytokinin receptors AHK3 and AHK4. This strongly implies that the effect of compound 82 is due to the up-regulation of cytokinin signalling. Overall, this study identifies highly effective and easily prepared CKX inhibitors with a low risk of environmental toxicity for further investigation of their potential in agriculture and biotechnology.

PubMed: 38776394
DOI: 10.1093/jxb/erae239

実験手法

X-RAY DIFFRACTION (1.93 Å)