8CFF

Crystal structure of arsenite oxidase from Alcaligenes faecalis (Af Aio) bound to arsenite

8CFF の概要

• エントリーDOI: 10.2210/pdb8cff/pdb
• 分子名称: Arsenite oxidase subunit AioA, DI(HYDROXYETHYL)ETHER, ISOPROPYL ALCOHOL, ... (13 entities in total)
• 機能のキーワード: arsenite oxidase, molybdenum cofactor, mgd, arsenic oxyanion, antimony oxyanion, oxidoreductase
• 由来する生物種: Alcaligenes faecalis; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 437552.02

構造登録者

Engrola, F.,Correia, M.A.S.,Romao, M.J.,Santos-Silva, T. (登録日: 2023-02-03, 公開日: 2023-07-12, 最終更新日: 2024-10-23)

主引用文献

Engrola, F.,Correia, M.A.S.,Watson, C.,Romao, C.C.,Veiros, L.F.,Romao, M.J.,Santos-Silva, T.,Santini, J.M.
Arsenite oxidase in complex with antimonite and arsenite oxyanions: Insights into the catalytic mechanism.
J.Biol.Chem., 299:105036-105036, 2023

PubMed Abstract: Arsenic contamination of groundwater is among one of the biggest health threats affecting millions of people in the world. There is an urgent need for efficient arsenic biosensors where the use of arsenic metabolizing enzymes can be explored. In this work, we have solved four crystal structures of arsenite oxidase (Aio) in complex with arsenic and antimony oxyanions and the structures determined correspond to intermediate states of the enzymatic mechanism. These structural data were complemented with density-functional theory calculations providing a unique view of the molybdenum active site at different time points that, together with mutagenesis data, enabled to clarify the enzymatic mechanism and the molecular determinants for the oxidation of As(III) to the less toxic As(V) species.

PubMed: 37442232
DOI: 10.1016/j.jbc.2023.105036

実験手法

X-RAY DIFFRACTION (1.57 Å)