8CF7

Dimethylated RSL-R5 in complex with cucurbit[7]uril, C121 sheet assembly

Summary for 8CF7

• Entry DOI: 10.2210/pdb8cf7/pdb
• Related: 8CF6
• Descriptor: RSL-R5, cucurbit[7]uril, GLYCEROL, ... (5 entities in total)
• Functional Keywords: lectin, cucurbituril, biomaterials, supramolecular, sugar binding protein
• Biological source: Ralstonia solanacearum
• Total number of polymer chains: 6
• Total formula weight: 67473.30

Authors

Ramberg, K.,Crowley, P.B. (deposition date: 2023-02-02, release date: 2023-07-05)

Primary citation

Ramberg, K.O.,Crowley, P.B.
Cage versus sheet: Probing the Determinants of Protein - Cucurbit[7]uril Crystalline Architectures.
J.Struct.Biol., 215:107969-107969, 2023

PubMed Abstract: The donut-shaped cucurbit[n]urils (Qn) are a class of rigid macrocyclic receptor with protein recognition capabilities. Qn encapsulation of amino acid side chains can enable protein assembly. Recently, cucurbit[7]uril (Q7) has been applied as a molecular glue for organizing protein building blocks into crystalline architectures. Q7 co-crystallization with dimethylated Ralstonia solanacearum lectin (RSL*) has yielded novel crystalline architectures. Co-crystallization of RSL* and Q7 yields either cage- or sheet-like architectures which may be modulated via protein engineering. However, questions remain as to the factors dictating the formation of one architecture over another (cage versus sheet). Here, we make use of an engineered RSL*-Q7 system which co-crystallizes as the cage or sheet assembly with easily-distinguished crystal morphologies. Using this model system, we probe how the crystallization conditions dictate which crystalline architecture is adopted. Protein-ligand ratios and the sodium concentration were identified as key determinants for the growth of the cage versus sheet assemblies.

PubMed: 37137399
DOI: 10.1016/j.jsb.2023.107969

Experimental method

X-RAY DIFFRACTION (1.14 Å)