8CDA
Crystal structure of MAB_4123 from Mycobacterium abscessus
Summary for 8CDA
| Entry DOI | 10.2210/pdb8cda/pdb |
| Descriptor | Probable monooxygenase, PHOSPHATE ION, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | monooxygenase, organosulfur compounds, flavin binding protein, unknown function |
| Biological source | Mycobacteroides abscessus ATCC 19977 |
| Total number of polymer chains | 16 |
| Total formula weight | 738503.01 |
| Authors | Ung, K.L.,Poussineau, C.,Couston, J.,Alsarraf, H.M.A.B.,Blaise, M. (deposition date: 2023-01-30, release date: 2023-05-10, Last modification date: 2024-06-19) |
| Primary citation | Ung, K.L.,Poussineau, C.,Couston, J.,Alsarraf, H.M.A.B.,Blaise, M. Crystal structure of MAB_4123, a putative flavin-dependent monooxygenase from Mycobacterium abscessus. Acta Crystallogr.,Sect.F, 79:128-136, 2023 Cited by PubMed Abstract: Numerous bacteria from different phylae can perform desulfurization reactions of organosulfur compounds. In these degradation or detoxification pathways, two-component flavin-dependent monooxygenases that use flavins (FMN or FAD) as a cofactor play important roles as they catalyse the first steps of these metabolic routes. The TdsC or DszC and MsuC proteins belong to this class of enzymes as they process dibenzothiophene (DBT) and methanesulfinate. Elucidation of their X-ray structures in apo, ligand-bound and cofactor-bound forms has provided important molecular insights into their catalytic reaction. Mycobacterial species have also been shown to possess a DBT degradation pathway, but no structural information is available on these two-component flavin-dependent monooxygenases. In this study, the crystal structure of the uncharacterized MAB_4123 protein from the human pathogen Mycobacterium abscessus is presented. The structure solved at high resolution displays high similarity to homologs from Rhodococcus, Paenibacillus and Pseudomonas species. In silico docking approaches suggest that MAB_4123 binds FMN and may use it as a cofactor. Structural analysis strongly suggests that MAB_4123 is a two-component flavin-dependent monooxygenase that could act as a detoxifying enzyme of organosulfur compounds in mycobacteria. PubMed: 37132477DOI: 10.1107/S2053230X2300345X PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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