8CDA

Crystal structure of MAB_4123 from Mycobacterium abscessus

8CDA の概要

• エントリーDOI: 10.2210/pdb8cda/pdb
• 分子名称: Probable monooxygenase, PHOSPHATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: monooxygenase, organosulfur compounds, flavin binding protein, unknown function
• 由来する生物種: Mycobacteroides abscessus ATCC 19977
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 738503.01

構造登録者

Ung, K.L.,Poussineau, C.,Couston, J.,Alsarraf, H.M.A.B.,Blaise, M. (登録日: 2023-01-30, 公開日: 2023-05-10, 最終更新日: 2024-06-19)

主引用文献

Ung, K.L.,Poussineau, C.,Couston, J.,Alsarraf, H.M.A.B.,Blaise, M.
Crystal structure of MAB_4123, a putative flavin-dependent monooxygenase from Mycobacterium abscessus.
Acta Crystallogr.,Sect.F, 79:128-136, 2023

PubMed Abstract: Numerous bacteria from different phylae can perform desulfurization reactions of organosulfur compounds. In these degradation or detoxification pathways, two-component flavin-dependent monooxygenases that use flavins (FMN or FAD) as a cofactor play important roles as they catalyse the first steps of these metabolic routes. The TdsC or DszC and MsuC proteins belong to this class of enzymes as they process dibenzothiophene (DBT) and methanesulfinate. Elucidation of their X-ray structures in apo, ligand-bound and cofactor-bound forms has provided important molecular insights into their catalytic reaction. Mycobacterial species have also been shown to possess a DBT degradation pathway, but no structural information is available on these two-component flavin-dependent monooxygenases. In this study, the crystal structure of the uncharacterized MAB_4123 protein from the human pathogen Mycobacterium abscessus is presented. The structure solved at high resolution displays high similarity to homologs from Rhodococcus, Paenibacillus and Pseudomonas species. In silico docking approaches suggest that MAB_4123 binds FMN and may use it as a cofactor. Structural analysis strongly suggests that MAB_4123 is a two-component flavin-dependent monooxygenase that could act as a detoxifying enzyme of organosulfur compounds in mycobacteria.

PubMed: 37132477
DOI: 10.1107/S2053230X2300345X

実験手法

X-RAY DIFFRACTION (2.1 Å)