8C9C

Cryo-EM captures early ribosome assembly in action

8C9C の概要

• エントリーDOI: 10.2210/pdb8c9c/pdb
• EMDBエントリー: 16509
• 分子名称: 23S rRNA, 50S ribosomal protein L22, 50S ribosomal protein L24, ... (4 entities in total)
• 機能のキーワード: ribosome, ribosome assembly, ribosome biogenesis, total reconstitution, rna, ribosomal protein.
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 972231.07

構造登録者

Nikolay, R.,Qin, B.,Lauer, S. (登録日: 2023-01-21, 公開日: 2023-04-05, 最終更新日: 2024-07-24)

主引用文献

Qin, B.,Lauer, S.M.,Balke, A.,Vieira-Vieira, C.H.,Burger, J.,Mielke, T.,Selbach, M.,Scheerer, P.,Spahn, C.M.T.,Nikolay, R.
Cryo-EM captures early ribosome assembly in action.
Nat Commun, 14:898-898, 2023

PubMed Abstract: Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) and ribosomal proteins. To obtain insights into the still unexplored early assembly phase of the bacterial 50S subunit, we exploited a minimal in vitro reconstitution system using purified ribosomal components and scalable reaction conditions. Time-limited assembly assays combined with cryo-EM analysis visualizes the structurally complex assembly pathway starting with a particle consisting of ordered density for only ~500 nucleotides of 23S rRNA domain I and three ribosomal proteins. In addition, our structural analysis reveals that early 50S assembly occurs in a domain-wise fashion, while late 50S assembly proceeds incrementally. Furthermore, we find that both ribosomal proteins and folded rRNA helices, occupying surface exposed regions on pre-50S particles, induce, or stabilize rRNA folds within adjacent regions, thereby creating cooperativity.

PubMed: 36797249
DOI: 10.1038/s41467-023-36607-9

実験手法

ELECTRON MICROSCOPY (6.62 Å)