8C8G

Cryo-EM structure of BoNT/Wo-NTNH complex

8C8G の概要

• エントリーDOI: 10.2210/pdb8c8g/pdb
• EMDBエントリー: 16475
• 分子名称: Putative botulinum-like toxin Wo, Structural protein (2 entities in total)
• 機能のキーワード: the complex of botulinum neurotoxin-like protein from weissella oryzae and its non-toxic non-hemagglutinin partner., toxin
• 由来する生物種: Weissella oryzae; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 323934.22

構造登録者

Kosenina, S.,Skerlova, J.,Stenmark, P. (登録日: 2023-01-20, 公開日: 2023-10-04, 最終更新日: 2024-10-23)

主引用文献

Kosenina, S.,Skerlova, J.,Zhang, S.,Dong, M.,Stenmark, P.
The cryo-EM structure of the BoNT/Wo-NTNH complex reveals two immunoglobulin-like domains.
Febs J., 291:676-689, 2024

PubMed Abstract: The botulinum neurotoxin-like toxin from Weissella oryzae (BoNT/Wo) is one of the BoNT-like toxins recently identified outside of the Clostridium genus. We show that, like the canonical BoNTs, BoNT/Wo forms a complex with its non-toxic non-hemagglutinin (NTNH) partner, which in traditional BoNT serotypes protects the toxin from proteases and the acidic environment of the hosts' guts. We here report the cryo-EM structure of the 300 kDa BoNT/Wo-NTNH/Wo complex together with pH stability studies of the complex. The structure reveals molecular details of the toxin's interactions with its protective partner. The overall structural arrangement is similar to other reported BoNT-NTNH complexes, but NTNH/Wo uniquely contains two extra bacterial immunoglobulin-like (Big) domains on the C-terminus. Although the function of these Big domains is unknown, they are structurally most similar to bacterial proteins involved in adhesion to host cells. In addition, the BoNT/Wo protease domain contains an internal disulfide bond not seen in other BoNTs. Mass photometry analysis revealed that the BoNT/Wo-NTNH/Wo complex is stable under acidic conditions and may dissociate at neutral to basic pH. These findings established that BoNT/Wo-NTNH/Wo shares the general fold of canonical BoNT-NTNH complexes. The presence of unique structural features suggests that it may have an alternative mode of activation, translocation and recognition of host cells, raising interesting questions about the activity and the mechanism of action of BoNT/Wo as well as about its target environment, receptors and substrates.

PubMed: 37746829
DOI: 10.1111/febs.16964

実験手法

ELECTRON MICROSCOPY (2.98 Å)