8C7J

Phage display derived serum albumin binding knob domain engineered within a novel VH framework 3 bispecific antibody format

Summary for 8C7J

• Entry DOI: 10.2210/pdb8c7j/pdb
• Descriptor: Fab heavy chain with knob domain, Fab light chain, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: fab knob domain hsa-binding domain, immune system
• Biological source: Cricetulus griseus; More
• Total number of polymer chains: 2
• Total formula weight: 52654.88

Authors

Adams, R.,Macpherson, A. (deposition date: 2023-01-16, release date: 2023-06-07, Last modification date: 2024-10-16)

Primary citation

Adams, R.,Joyce, C.,Kuravskiy, M.,Harrison, K.,Ahdash, Z.,Balmforth, M.,Chia, K.,Marceddu, C.,Coates, M.,Snowden, J.,Goursaud, E.,Menochet, K.,van den Elsen, J.,Payne, R.J.,Lawson, A.D.G.,Scott-Tucker, A.,Macpherson, A.
Serum albumin binding knob domains engineered within a V H framework III bispecific antibody format and as chimeric peptides.
Front Immunol, 14:1170357-1170357, 2023

PubMed Abstract: Serum albumin binding is an established mechanism to extend the serum half-life of antibody fragments and peptides. The cysteine rich knob domains, isolated from bovine antibody ultralong CDRH3, are the smallest single chain antibody fragments described to date and versatile tools for protein engineering.

PubMed: 37251411
DOI: 10.3389/fimmu.2023.1170357

Experimental method

X-RAY DIFFRACTION (2 Å)