8C7C

Double mutant V(M84)C/A(L278)C structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV

8C7C の概要

• エントリーDOI: 10.2210/pdb8c7c/pdb
• 分子名称: Reaction center protein H chain, CARDIOLIPIN, FE (III) ION, ... (14 entities in total)
• 機能のキーワード: photosynthetic reaction center, bacteriochlorophyll, rhodobacter sphaeroides, spheroidene, ubiquinone, disulfide bond, photosynthesis
• 由来する生物種: Cereibacter sphaeroides 2.4.1; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 102563.08

構造登録者

Gabdulkhakov, A.,Selikhanov, G.,Fufina, T.,Vasilieva, L.,Atamas, A.,Uhimchuk, D. (登録日: 2023-01-14, 公開日: 2023-11-22, 最終更新日: 2024-11-06)

主引用文献

Selikhanov, G.,Atamas, A.,Yukhimchuk, D.,Fufina, T.,Vasilieva, L.,Gabdulkhakov, A.
Stabilization of Cereibacter sphaeroides Photosynthetic Reaction Center by the Introduction of Disulfide Bonds.
Membranes (Basel), 13:-, 2023

PubMed Abstract: The photosynthetic reaction center of the purple nonsulfur bacterium is a useful model for the study of mechanisms of photoinduced electron transfer and a promising component for photo-bio-electrocatalytic systems. The basic research and technological applications of this membrane pigment-protein complex require effective approaches to increase its structural stability. In this work, a rational design approach to genetically modify the reaction centers by introducing disulfide bonds is used. This resulted in significantly increasing the thermal stability of some of the mutant pigment-protein complexes. The formation of the S-S bonds was confirmed by X-ray crystallography as well as SDS-PAGE, and the optical properties of the reaction centers were studied. The genetically modified reaction centers presented here preserved their ability for photochemical charge separation and could be of interest for basic science and biotechnology.

PubMed: 36837657
DOI: 10.3390/membranes13020154

実験手法

X-RAY DIFFRACTION (2.6 Å)