8C5J

Spatial structure of Lch-alpha peptide from two-component lantibiotic system Lichenicidin VK21

Replaces:  2KTN

Summary for 8C5J

• Entry DOI: 10.2210/pdb8c5j/pdb
• Related: 2KTO
• NMR Information: BMRB: 16707,16709
• Descriptor: Lantibiotic lichenicidin VK21 A1 (1 entity in total)
• Functional Keywords: lichenicidin vk21, lantibiotics, lcha, antibiotic, lanthionine, thioester, two-component lantibiotic system
• Biological source: Bacillus licheniformis
• Total number of polymer chains: 1
• Total formula weight: 3261.92

Authors

Mineev, K.S.,Paramonov, A.S.,Arseniev, A.S.,Ovchinnikova, T.V.,Shenkarev, Z.O. (deposition date: 2023-01-09, release date: 2023-03-22, Last modification date: 2023-11-15)

Primary citation

Panina, I.S.,Balandin, S.V.,Tsarev, A.V.,Chugunov, A.O.,Tagaev, A.A.,Finkina, E.I.,Antoshina, D.V.,Sheremeteva, E.V.,Paramonov, A.S.,Rickmeyer, J.,Bierbaum, G.,Efremov, R.G.,Shenkarev, Z.O.,Ovchinnikova, T.V.
Specific Binding of the alpha-Component of the Lantibiotic Lichenicidin to the Peptidoglycan Precursor Lipid II Predetermines Its Antimicrobial Activity.
Int J Mol Sci, 24:-, 2023

PubMed Abstract: To date, a number of lantibiotics have been shown to use lipid II-a highly conserved peptidoglycan precursor in the cytoplasmic membrane of bacteria-as their molecular target. The α-component (Lchα) of the two-component lantibiotic lichenicidin, previously isolated from the VK21 strain, seems to contain two putative lipid II binding sites in its -terminal and -terminal domains. Using NMR spectroscopy in DPC micelles, we obtained convincing evidence that the -terminal mersacidin-like site is involved in the interaction with lipid II. These data were confirmed by the MD simulations. The contact area of lipid II includes pyrophosphate and disaccharide residues along with the first isoprene units of bactoprenol. MD also showed the potential for the formation of a stable -terminal nisin-like complex; however, the conditions necessary for its implementation in vitro remain unknown. Overall, our results clarify the picture of two component lantibiotics mechanism of antimicrobial action.

PubMed: 36674846
DOI: 10.3390/ijms24021332

Experimental method

SOLUTION NMR