8C5J

Spatial structure of Lch-alpha peptide from two-component lantibiotic system Lichenicidin VK21

「2KTN」から置き換えられました

8C5J の概要

• エントリーDOI: 10.2210/pdb8c5j/pdb
• 関連するPDBエントリー: 2KTO
• NMR情報: BMRB: 16707,16709
• 分子名称: Lantibiotic lichenicidin VK21 A1 (1 entity in total)
• 機能のキーワード: lichenicidin vk21, lantibiotics, lcha, antibiotic, lanthionine, thioester, two-component lantibiotic system
• 由来する生物種: Bacillus licheniformis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3261.92

構造登録者

Mineev, K.S.,Paramonov, A.S.,Arseniev, A.S.,Ovchinnikova, T.V.,Shenkarev, Z.O. (登録日: 2023-01-09, 公開日: 2023-03-22, 最終更新日: 2023-11-15)

主引用文献

Panina, I.S.,Balandin, S.V.,Tsarev, A.V.,Chugunov, A.O.,Tagaev, A.A.,Finkina, E.I.,Antoshina, D.V.,Sheremeteva, E.V.,Paramonov, A.S.,Rickmeyer, J.,Bierbaum, G.,Efremov, R.G.,Shenkarev, Z.O.,Ovchinnikova, T.V.
Specific Binding of the alpha-Component of the Lantibiotic Lichenicidin to the Peptidoglycan Precursor Lipid II Predetermines Its Antimicrobial Activity.
Int J Mol Sci, 24:-, 2023

PubMed Abstract: To date, a number of lantibiotics have been shown to use lipid II-a highly conserved peptidoglycan precursor in the cytoplasmic membrane of bacteria-as their molecular target. The α-component (Lchα) of the two-component lantibiotic lichenicidin, previously isolated from the VK21 strain, seems to contain two putative lipid II binding sites in its -terminal and -terminal domains. Using NMR spectroscopy in DPC micelles, we obtained convincing evidence that the -terminal mersacidin-like site is involved in the interaction with lipid II. These data were confirmed by the MD simulations. The contact area of lipid II includes pyrophosphate and disaccharide residues along with the first isoprene units of bactoprenol. MD also showed the potential for the formation of a stable -terminal nisin-like complex; however, the conditions necessary for its implementation in vitro remain unknown. Overall, our results clarify the picture of two component lantibiotics mechanism of antimicrobial action.

PubMed: 36674846
DOI: 10.3390/ijms24021332

実験手法

SOLUTION NMR