8C4A

Structural and interactional insights into the glideosome-associated connector from Toxoplasma gondii

8C4A の概要

• エントリーDOI: 10.2210/pdb8c4a/pdb
• 分子名称: Putative anonymous antigen-1 (2 entities in total)
• 機能のキーワード: glideosome-associated connector (gac), toxoplasma gondii, structural protein
• 由来する生物種: Toxoplasma gondii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 274375.75

構造登録者

Kumar, A.,Morgan, R.M.L.,Matthews, S.J. (登録日: 2023-01-03, 公開日: 2023-07-19)

主引用文献

Kumar, A.,Vadas, O.,Dos Santos Pacheco, N.,Zhang, X.,Chao, K.,Darvill, N.,Rasmussen, H.O.,Xu, Y.,Lin, G.M.,Stylianou, F.A.,Pedersen, J.S.,Rouse, S.L.,Morgan, M.L.,Soldati-Favre, D.,Matthews, S.
Structural and regulatory insights into the glideosome-associated connector from Toxoplasma gondii.
Elife, 12:-, 2023

PubMed Abstract: The phylum of Apicomplexa groups intracellular parasites that employ substrate-dependent gliding motility to invade host cells, egress from the infected cells, and cross biological barriers. The glideosome-associated connector (GAC) is a conserved protein essential to this process. GAC facilitates the association of actin filaments with surface transmembrane adhesins and the efficient transmission of the force generated by myosin translocation of actin to the cell surface substrate. Here, we present the crystal structure of GAC and reveal a unique, supercoiled armadillo repeat region that adopts a closed ring conformation. Characterisation of the solution properties together with membrane and F-actin binding interfaces suggests that GAC adopts several conformations from closed to open and extended. A multi-conformational model for assembly and regulation of GAC within the glideosome is proposed.

PubMed: 37014051
DOI: 10.7554/eLife.86049

実験手法

X-RAY DIFFRACTION (2.675 Å)