8C20

Tetrameric 5-HT3aR in Salipro (holo state, symmetric)

8C20 の概要

• エントリーDOI: 10.2210/pdb8c20/pdb
• 関連するPDBエントリー: 8C1Z
• EMDBエントリー: 16386
• 分子名称: 5-hydroxytryptamine receptor 3A, SEROTONIN (2 entities in total)
• 機能のキーワード: 5-ht3r, serotonin, receptor, tetramer, ion channel, membrane protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 243024.77

構造登録者

Introini, B.,Kudryashev, M. (登録日: 2022-12-21, 公開日: 2024-07-10, 最終更新日: 2024-10-23)

主引用文献

Introini, B.,Cui, W.,Chu, X.,Zhang, Y.,Alves, A.C.,Eckhardt-Strelau, L.,Golusik, S.,Tol, M.,Vogel, H.,Yuan, S.,Kudryashev, M.
Structure of tetrameric forms of the serotonin-gated 5-HT3 A receptor ion channel.
Embo J., 43:4451-4471, 2024

PubMed Abstract: Multimeric membrane proteins are produced in the endoplasmic reticulum and transported to their target membranes which, for ion channels, is typically the plasma membrane. Despite the availability of many fully assembled channel structures, our understanding of assembly intermediates, multimer assembly mechanisms, and potential functions of non-standard assemblies is limited. We demonstrate that the pentameric ligand-gated serotonin 5-HT3A receptor (5-HT3AR) can assemble to tetrameric forms and report the structures of the tetramers in plasma membranes of cell-derived microvesicles and in membrane memetics using cryo-electron microscopy and tomography. The tetrameric structures have near-symmetric transmembrane domains, and asymmetric extracellular domains, and can bind serotonin molecules. Computer simulations, based on our cryo-EM structures, were used to decipher the assembly pathway of pentameric 5-HT3R and suggest a potential functional role for the tetrameric receptors.

PubMed: 39232129
DOI: 10.1038/s44318-024-00191-5

実験手法

ELECTRON MICROSCOPY (3.5 Å)