8BXT

Structure of StayGold

Summary for 8BXT

Entry DOI	10.2210/pdb8bxt/pdb
Descriptor	StayGold, CHLORIDE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
Functional Keywords	dimer, fluorescent protein
Biological source	Cytaeis uchidae
Total number of polymer chains	2
Total formula weight	50066.85
Authors	Ivorra-Molla, E.,Akhuli, D.,Crow, A. (deposition date: 2022-12-09, release date: 2023-07-19, Last modification date: 2024-10-23)
Primary citation	Ivorra-Molla, E.,Akhuli, D.,McAndrew, M.B.L.,Scott, W.,Kumar, L.,Palani, S.,Mishima, M.,Crow, A.,Balasubramanian, M.K. A monomeric StayGold fluorescent protein. Nat.Biotechnol., 42:1368-1371, 2024 Cited by PubMed Abstract: StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric. PubMed: 38081970 DOI: 10.1038/s41587-023-02018-w PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.6 Å)

Structure validation