8BXT

Structure of StayGold

8BXT の概要

• エントリーDOI: 10.2210/pdb8bxt/pdb
• 分子名称: StayGold, CHLORIDE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: dimer, fluorescent protein
• 由来する生物種: Cytaeis uchidae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50066.85

構造登録者

Ivorra-Molla, E.,Akhuli, D.,Crow, A. (登録日: 2022-12-09, 公開日: 2023-07-19, 最終更新日: 2024-10-23)

主引用文献

Ivorra-Molla, E.,Akhuli, D.,McAndrew, M.B.L.,Scott, W.,Kumar, L.,Palani, S.,Mishima, M.,Crow, A.,Balasubramanian, M.K.
A monomeric StayGold fluorescent protein.
Nat.Biotechnol., 42:1368-1371, 2024

PubMed Abstract: StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.

PubMed: 38081970
DOI: 10.1038/s41587-023-02018-w

実験手法

X-RAY DIFFRACTION (1.6 Å)