8BXL

Patulin Synthase from Penicillium expansum

8BXL の概要

• エントリーDOI: 10.2210/pdb8bxl/pdb
• 分子名称: Patulin synthase, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: oxidoreductase, gmc-type flavoprotein, biocatalysis, flavoprotein
• 由来する生物種: Penicillium expansum
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 425335.91

構造登録者

Tjallinks, G.,Boverio, A.,Rozeboom, H.J.,Fraaije, M.W. (登録日: 2022-12-09, 公開日: 2023-09-06, 最終更新日: 2024-10-23)

主引用文献

Tjallinks, G.,Boverio, A.,Maric, I.,Rozeboom, H.,Arentshorst, M.,Visser, J.,Ram, A.F.J.,Mattevi, A.,Fraaije, M.W.
Structure elucidation and characterization of patulin synthase, insights into the formation of a fungal mycotoxin.
Febs J., 290:5114-5126, 2023

PubMed Abstract: Patulin synthase (PatE) from Penicillium expansum is a flavin-dependent enzyme that catalyses the last step in the biosynthesis of the mycotoxin patulin. This secondary metabolite is often present in fruit and fruit-derived products, causing postharvest losses. The patE gene was expressed in Aspergillus niger allowing purification and characterization of PatE. This confirmed that PatE is active not only on the proposed patulin precursor ascladiol but also on several aromatic alcohols including 5-hydroxymethylfurfural. By elucidating its crystal structure, details on its catalytic mechanism were revealed. Several aspects of the active site architecture are reminiscent of that of fungal aryl-alcohol oxidases. Yet, PatE is most efficient with ascladiol as substrate confirming its dedicated role in biosynthesis of patulin.

PubMed: 37366079
DOI: 10.1111/febs.16804

実験手法

X-RAY DIFFRACTION (2.4 Å)