8BXF

Alvinella pompejana nicotinic acetylcholine receptor Alpo4 in apo state (Alpo4_apo, dataset 1)

Summary for 8BXF

• Entry DOI: 10.2210/pdb8bxf/pdb
• EMDB information: 16317
• Descriptor: Acetylcholine receptor, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• Functional Keywords: cys-loop receptor, pentameric ligand-gated ion channel, alpo, nachr, membrane protein
• Biological source: Alvinella pompejana
• Total number of polymer chains: 5
• Total formula weight: 271880.71

Authors

De Gieter, S.,Efremov, R.G.,Ulens, C. (deposition date: 2022-12-08, release date: 2023-07-12)

Primary citation

De Gieter, S.,Gallagher, C.,Wijckmans, E.,Pasini, D.,Ulens, C.,Efremov, R.G.
Sterol derivative binding to the orthosteric site causes conformational changes in an invertebrate Cys-loop receptor.
Elife, 12:-, 2023

PubMed Abstract: Cys-loop receptors or pentameric ligand-gated ion channels are mediators of electrochemical signaling throughout the animal kingdom. Because of their critical function in neurotransmission and high potential as drug targets, Cys-loop receptors from humans and closely related organisms have been thoroughly investigated, whereas molecular mechanisms of neurotransmission in invertebrates are less understood. When compared with vertebrates, the invertebrate genomes underwent a drastic expansion in the number of the nACh-like genes associated with receptors of unknown function. Understanding this diversity contributes to better insight into the evolution and possible functional divergence of these receptors. In this work, we studied orphan receptor Alpo4 from an extreme thermophile worm . Sequence analysis points towards its remote relation to characterized nACh receptors. We solved the cryo-EM structure of the lophotrochozoan nACh-like receptor in which a CHAPS molecule is tightly bound to the orthosteric site. We show that the binding of CHAPS leads to extending of the loop C at the orthosteric site and a quaternary twist between extracellular and transmembrane domains. Both the ligand binding site and the channel pore reveal unique features. These include a conserved Trp residue in loop B of the ligand binding site which is flipped into an apparent self-liganded state in the apo structure. The ion pore of Alpo4 is tightly constricted by a ring of methionines near the extracellular entryway of the channel pore. Our data provide a structural basis for a functional understanding of Alpo4 and hints towards new strategies for designing specific channel modulators.

PubMed: 37395731
DOI: 10.7554/eLife.86029

Experimental method

ELECTRON MICROSCOPY (3.4 Å)