8BUU の概要
| エントリーDOI | 10.2210/pdb8buu/pdb |
| EMDBエントリー | 16246 |
| 分子名称 | 23S rRNA, 50S ribosomal protein L15, 50S ribosomal protein L16, ... (55 entities in total) |
| 機能のキーワード | abc, abcf, abc-f, vmlr2, antibiotic resistance, initiation, distorted p-trna, shina-dalgarno, neobacillus vireti, bacillus subtilis, ribosome |
| 由来する生物種 | Neobacillus vireti 詳細 |
| タンパク質・核酸の鎖数 | 51 |
| 化学式量合計 | 2207148.99 |
| 構造登録者 | |
| 主引用文献 | Obana, N.,Takada, H.,Crowe-McAuliffe, C.,Iwamoto, M.,Egorov, A.A.,Wu, K.J.Y.,Chiba, S.,Murina, V.,Paternoga, H.,Tresco, B.I.C.,Nomura, N.,Myers, A.G.,Atkinson, G.C.,Wilson, D.N.,Hauryliuk, V. Genome-encoded ABCF factors implicated in intrinsic antibiotic resistance in Gram-positive bacteria: VmlR2, Ard1 and CplR. Nucleic Acids Res., 51:4536-4554, 2023 Cited by PubMed Abstract: Genome-encoded antibiotic resistance (ARE) ATP-binding cassette (ABC) proteins of the F subfamily (ARE-ABCFs) mediate intrinsic resistance in diverse Gram-positive bacteria. The diversity of chromosomally-encoded ARE-ABCFs is far from being fully experimentally explored. Here we characterise phylogenetically diverse genome-encoded ABCFs from Actinomycetia (Ard1 from Streptomyces capreolus, producer of the nucleoside antibiotic A201A), Bacilli (VmlR2 from soil bacterium Neobacillus vireti) and Clostridia (CplR from Clostridium perfringens, Clostridium sporogenes and Clostridioides difficile). We demonstrate that Ard1 is a narrow spectrum ARE-ABCF that specifically mediates self-resistance against nucleoside antibiotics. The single-particle cryo-EM structure of a VmlR2-ribosome complex allows us to rationalise the resistance spectrum of this ARE-ABCF that is equipped with an unusually long antibiotic resistance determinant (ARD) subdomain. We show that CplR contributes to intrinsic pleuromutilin, lincosamide and streptogramin A resistance in Clostridioides, and demonstrate that C. difficile CplR (CDIF630_02847) synergises with the transposon-encoded 23S ribosomal RNA methyltransferase Erm to grant high levels of antibiotic resistance to the C. difficile 630 clinical isolate. Finally, assisted by uORF4u, our novel tool for detection of upstream open reading frames, we dissect the translational attenuation mechanism that controls the induction of cplR expression upon an antibiotic challenge. PubMed: 36951104DOI: 10.1093/nar/gkad193 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (2.9 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
