8BTM

Structural and functional studies of geldanamycin amide synthase ShGdmF

8BTM の概要

• エントリーDOI: 10.2210/pdb8btm/pdb
• 分子名称: GdmF, 1,2-ETHANEDIOL, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: amide synthase, geldanamycin, amidase, alpha-beta, biosynthetic protein
• 由来する生物種: Streptomyces hygroscopicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30176.78

構造登録者

Ewert, W.,Zeilinger, C.,Kirschning, A.,Preller, M. (登録日: 2022-11-29, 公開日: 2024-06-12, 最終更新日: 2025-04-02)

主引用文献

Ewert, W.,Bartens, C.,Ongouta, J.,Holmes, M.,Heutling, A.,Kishore, A.,Urbansky, T.,Zeilinger, C.,Preller, M.,Kirschning, A.
Structure and function of the geldanamycin amide synthase from Streptomyces hygroscopicus.
Nat Commun, 16:2464-2464, 2025

PubMed Abstract: Amide synthases catalyze the formation of macrolactam rings from aniline-containing polyketide-derived seco-acids as found in the important class of ansamycin antibiotics. One of these amide synthases is the geldanamycin amide synthase GdmF, which we recombinantly expressed, purified and studied in detail both functionally as well as structurally. Here we show that purified GdmF catalyzes the amide formation using synthetically derived substrates. The atomic structures of the ligand-free enzyme and in complex with simplified substrates reveal distinct structural features of the substrate binding site and a putative role of the flexible interdomain region for the catalysis reaction.

PubMed: 40075103
DOI: 10.1038/s41467-025-57013-3

実験手法

X-RAY DIFFRACTION (1.4 Å)