8BQB

CjCel5C endo-glucanase bound to CB396 covalent inhibitor

8BQB の概要

• エントリーDOI: 10.2210/pdb8bqb/pdb
• 分子名称: Cellulase, putative, cel5C, (1R,2S,3S,4S,5R,6R)-6-(HYDROXYMETHYL)CYCLOHEXANE-1,2,3,4,5-PENTOL, beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: carbohydrate, inhibitor, covalent, cellulase, hydrolase
• 由来する生物種: Cellvibrio japonicus Ueda107
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 121728.84

構造登録者

McGregor, N.G.S.,de Boer, C.,Overkleeft, H.S.,Davies, G.J. (登録日: 2022-11-20, 公開日: 2023-11-29, 最終更新日: 2024-11-06)

主引用文献

McGregor, N.G.S.,de Boer, C.,Foucart, Q.P.O.,Beenakker, T.,Offen, W.A.,Codee, J.D.C.,Willems, L.I.,Overkleeft, H.S.,Davies, G.J.
A Multiplexing Activity-Based Protein-Profiling Platform for Dissection of a Native Bacterial Xyloglucan-Degrading System.
Acs Cent.Sci., 9:2306-2314, 2023

PubMed Abstract: Bacteria and yeasts grow on biomass polysaccharides by expressing and excreting a complex array of glycoside hydrolase (GH) enzymes. Identification and annotation of such GH pools, which are valuable commodities for sustainable energy and chemistries, by conventional means (genomics, proteomics) are complicated, as primary sequence or secondary structure alignment with known active enzymes is not always predictive for new ones. Here we report a "low-tech", easy-to-use, and sensitive multiplexing activity-based protein-profiling platform to characterize the xyloglucan-degrading GH system excreted by the soil saprophyte, , when grown on xyloglucan. A suite of activity-based probes bearing orthogonal fluorophores allows for the visualization of accessory -acting glycosidases, which are then identified using biotin-bearing probes. Substrate specificity of xyloglucanases is directly revealed by imbuing xyloglucan structural elements into bespoke activity-based probes. Our ABPP platform provides a highly useful tool to dissect xyloglucan-degrading systems from various sources and to rapidly select potentially useful ones. The observed specificity of the probes moreover bodes well for the study of other biomass polysaccharide-degrading systems, by modeling probe structures to those of desired substrates.

PubMed: 38161374
DOI: 10.1021/acscentsci.3c00831

実験手法

X-RAY DIFFRACTION (2.701 Å)