8BOZ

structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS effector/immunity complex

8BOZ の概要

• エントリーDOI: 10.2210/pdb8boz/pdb
• 分子名称: Transmembrane protein, Lipoprotein, CALCIUM ION (3 entities in total)
• 機能のキーワード: phospholipase, immunity, adherent-invasive escherichia coli (aiec), , protein secretion, protein structure, alphafold2, hydrolase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 837560.70

構造登録者

Cambillau, C.,Roussel, A. (登録日: 2022-11-15, 公開日: 2023-02-08, 最終更新日: 2024-11-20)

主引用文献

Le, T.T.H.,Kellenberger, C.,Boyer, M.,Santucci, P.,Flaugnatti, N.,Cascales, E.,Roussel, A.,Canaan, S.,Journet, L.,Cambillau, C.
Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS Effector/Immunity Complex Determined Using an AlphaFold2 Predicted Model.
Int J Mol Sci, 24:-, 2023

PubMed Abstract: The type VI secretion system (T6SS) delivers enzymatic effectors into target cells to destroy them. Cells of the same strain protect themselves against effectors with immunity proteins that specifically inhibit effectors. Here, we report the identification and characterization of a Tle3 phospholipase effector and its cognate immunity protein Tli3-an outer membrane lipoprotein from adherent-invasive (AIEC). Enzymatic assays demonstrate that purified Tle3 has a phospholipase A1, and not A2, activity and that its toxicity is neutralized by the cognate immunity protein Tli3. Tli3 binds Tle3 in a 1:1 stoichiometric ratio. Tle3, Tli3 and the Tle3-Tli3 complex were purified and subjected to crystallization. The Tle3-Tli3 complex structure could not be solved by SeMet phasing, but only by molecular replacement when using an AlphaFold2 prediction model. Tle3 exhibits an α/β-hydrolase fold decorated by two protruding segments, including a N-terminus loop. Tli3 displays a new fold of three stacked β-sheets and a protruding loop that inserts in Tle3catalytic crevice. We showed, experimentally, that Tle3 interacts with the VgrG cargo protein and AlphaFold2 prediction of the VgrG-Tle3 complex reveals a strong interaction between the VgrG C-terminus adaptor and Tle3 N-terminal loop.

PubMed: 36675258
DOI: 10.3390/ijms24021740

実験手法

X-RAY DIFFRACTION (3.6 Å)