8BON

Structure of the SARS-CoV-2 spike glycoprotein in complex with the macrocyclic peptide S1B3inL1

これはPDB形式変換不可エントリーです。

8BON の概要

• エントリーDOI: 10.2210/pdb8bon/pdb
• EMDBエントリー: 16144
• 分子名称: Spike glycoprotein,Fibritin, Macrocyclic peptide S1B3inL1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: glycoprotein, macrocyclic peptide, complex, inhibitor, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 440509.12

構造登録者

Hurdiss, D.L. (登録日: 2022-11-15, 公開日: 2023-06-28, 最終更新日: 2023-07-05)

主引用文献

Thijssen, V.,Hurdiss, D.L.,Debski-Antoniak, O.J.,Spence, M.A.,Franck, C.,Norman, A.,Aggarwal, A.,Mokiem, N.J.,van Dongen, D.A.A.,Vermeir, S.W.,Liu, M.,Li, W.,Chatziandreou, M.,Donselaar, T.,Du, W.,Drulyte, I.,Bosch, B.J.,Snijder, J.,Turville, S.G.,Payne, R.J.,Jackson, C.J.,van Kuppeveld, F.J.M.,Jongkees, S.A.K.
A broad-spectrum macrocyclic peptide inhibitor of the SARS-CoV-2 spike protein.
Proc.Natl.Acad.Sci.USA, 120:e2303292120-e2303292120, 2023

PubMed Abstract: The ongoing COVID-19 pandemic has had great societal and health consequences. Despite the availability of vaccines, infection rates remain high due to immune evasive Omicron sublineages. Broad-spectrum antivirals are needed to safeguard against emerging variants and future pandemics. We used messenger RNA (mRNA) display under a reprogrammed genetic code to find a spike-targeting macrocyclic peptide that inhibits SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) Wuhan strain infection and pseudoviruses containing spike proteins of SARS-CoV-2 variants or related sarbecoviruses. Structural and bioinformatic analyses reveal a conserved binding pocket between the receptor-binding domain, N-terminal domain, and S2 region, distal to the angiotensin-converting enzyme 2 receptor-interaction site. Our data reveal a hitherto unexplored site of vulnerability in sarbecoviruses that peptides and potentially other drug-like molecules can target.

PubMed: 37339194
DOI: 10.1073/pnas.2303292120

実験手法

ELECTRON MICROSCOPY (3.2 Å)