8BMV

Ligand binding domain of the P. Putida receptor McpH in complex with Uric acid

8BMV の概要

• エントリーDOI: 10.2210/pdb8bmv/pdb
• 分子名称: Methyl-accepting chemotaxis protein McpH, URIC ACID (3 entities in total)
• 機能のキーワード: ligand binding domain, chemoreceptor, pseudomonas putida, chemotactic transducer, signaling protein
• 由来する生物種: Pseudomonas putida KT2440
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61566.95

構造登録者

Gavira, J.A.,Krell, T.,Fernandez, M.,Martinez-Rodriguez, S. (登録日: 2022-11-11, 公開日: 2024-07-24)

主引用文献

Monteagudo-Cascales, E.,Gumerov, V.M.,Fernandez, M.,Matilla, M.A.,Gavira, J.A.,Zhulin, I.B.,Krell, T.
Ubiquitous purine sensor modulates diverse signal transduction pathways in bacteria.
Nat Commun, 15:5867-5867, 2024

PubMed Abstract: Purines and their derivatives control intracellular energy homeostasis and nucleotide synthesis, and act as signaling molecules. Here, we combine structural and sequence information to define a purine-binding motif that is present in sensor domains of thousands of bacterial receptors that modulate motility, gene expression, metabolism, and second-messenger turnover. Microcalorimetric titrations of selected sensor domains validate their ability to specifically bind purine derivatives, and evolutionary analyses indicate that purine sensors share a common ancestor with amino-acid receptors. Furthermore, we provide experimental evidence of physiological relevance of purine sensing in a second-messenger signaling system that modulates c-di-GMP levels.

PubMed: 38997289
DOI: 10.1038/s41467-024-50275-3

実験手法

X-RAY DIFFRACTION (1.95 Å)