8BIY

Citrate-free extracytoplasmic PAS domain mutant R93A of sensor histidine kinase CitA from Geobacillus thermodenitrificans

8BIY の概要

• エントリーDOI: 10.2210/pdb8biy/pdb
• 分子名称: Histidine kinase, 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: signal transduction, geobacillus thermodenitrificans, sensor histidine kinase, cita, sensor domain, extracytoplasmic pas domain, r93a mutation, two-component signaling system, signaling protein
• 由来する生物種: Geobacillus thermodenitrificans
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 57517.79

構造登録者

Becker, S. (登録日: 2022-11-02, 公開日: 2023-11-15, 最終更新日: 2026-03-04)

主引用文献

Zhang, X.C.,Xue, K.,Salvi, M.,Schomburg, B.,Mehrens, J.,Giller, K.,Stopp, M.,Weisenburger, S.,Boning, D.,Sandoghdar, V.,Unden, G.,Becker, S.,Andreas, L.B.,Griesinger, C.
Mechanism of sensor kinase CitA transmembrane signaling.
Nat Commun, 16:935-935, 2025

PubMed Abstract: Membrane bound histidine kinases (HKs) are ubiquitous sensors of extracellular stimuli in bacteria. However, a uniform structural model is still missing for their transmembrane signaling mechanism. Here, we used solid-state NMR in conjunction with crystallography, solution NMR and distance measurements to investigate the transmembrane signaling mechanism of a paradigmatic citrate sensing membrane embedded HK, CitA. Citrate binding in the sensory extracytoplasmic PAS domain (PASp) causes the linker to transmembrane helix 2 (TM2) to adopt a helical conformation. This triggers a piston-like pulling of TM2 and a quaternary structure rearrangement in the cytosolic PAS domain (PASc). Crystal structures of PASc reveal both anti-parallel and parallel dimer conformations. An anti-parallel to parallel transition upon citrate binding agrees with interdimer distances measured in the lipid embedded protein using a site-specific F label in PASc. These data show how Angstrom scale structural changes in the sensor domain are transmitted across the membrane to be converted and amplified into a nm scale shift in the linker to the phosphorylation subdomain of the kinase.

PubMed: 39843904
DOI: 10.1038/s41467-024-55671-3

実験手法

X-RAY DIFFRACTION (1.61 Å)