8BI4

Helical shell of CCMV capsid protein on DNA origami 6HB-2k

Summary for 8BI4

• Entry DOI: 10.2210/pdb8bi4/pdb
• EMDB information: 16076
• Descriptor: Coat protein (1 entity in total)
• Functional Keywords: origami, capsid, virus like particle
• Biological source: Cowpea chlorotic mottle virus
• Total number of polymer chains: 6
• Total formula weight: 122017.51

Authors

Kumpula, E.-P.,Seitz, I.,Kostiainen, M.A.,Huiskonen, J.T. (deposition date: 2022-11-01, release date: 2023-07-05, Last modification date: 2023-10-25)

Primary citation

Seitz, I.,Saarinen, S.,Kumpula, E.P.,McNeale, D.,Anaya-Plaza, E.,Lampinen, V.,Hytonen, V.P.,Sainsbury, F.,Cornelissen, J.J.L.M.,Linko, V.,Huiskonen, J.T.,Kostiainen, M.A.
DNA-origami-directed virus capsid polymorphism.
Nat Nanotechnol, 18:1205-1212, 2023

PubMed Abstract: Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA-DNA origami structures to pave way for next-generation cargo protection and targeting strategies.

PubMed: 37460794
DOI: 10.1038/s41565-023-01443-x

Experimental method

ELECTRON MICROSCOPY (4.3 Å)