8BI4

Helical shell of CCMV capsid protein on DNA origami 6HB-2k

8BI4 の概要

• エントリーDOI: 10.2210/pdb8bi4/pdb
• EMDBエントリー: 16076
• 分子名称: Coat protein (1 entity in total)
• 機能のキーワード: origami, capsid, virus like particle
• 由来する生物種: Cowpea chlorotic mottle virus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 122017.51

構造登録者

Kumpula, E.-P.,Seitz, I.,Kostiainen, M.A.,Huiskonen, J.T. (登録日: 2022-11-01, 公開日: 2023-07-05, 最終更新日: 2023-10-25)

主引用文献

Seitz, I.,Saarinen, S.,Kumpula, E.P.,McNeale, D.,Anaya-Plaza, E.,Lampinen, V.,Hytonen, V.P.,Sainsbury, F.,Cornelissen, J.J.L.M.,Linko, V.,Huiskonen, J.T.,Kostiainen, M.A.
DNA-origami-directed virus capsid polymorphism.
Nat Nanotechnol, 18:1205-1212, 2023

PubMed Abstract: Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA-DNA origami structures to pave way for next-generation cargo protection and targeting strategies.

PubMed: 37460794
DOI: 10.1038/s41565-023-01443-x

実験手法

ELECTRON MICROSCOPY (4.3 Å)