8BGH

Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAA mRNA codon in the A-site after uncompleted di-peptide formation

これはPDB形式変換不可エントリーです。

8BGH の概要

• エントリーDOI: 10.2210/pdb8bgh/pdb
• 関連するPDBエントリー: 8BF7 8BGE
• EMDBエントリー: 16015 16029 16031
• 分子名称: 50S ribosomal protein L2, 50S ribosomal protein L16, 50S ribosomal protein L17, ... (55 entities in total)
• 機能のキーワード: m6a, mrna modification, trna recognition, translation
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 54
• 化学式量合計: 2174247.20

構造登録者

Koziej, L.,Glatt, S. (登録日: 2022-10-27, 公開日: 2023-08-16)

主引用文献

Jain, S.,Koziej, L.,Poulis, P.,Kaczmarczyk, I.,Gaik, M.,Rawski, M.,Ranjan, N.,Glatt, S.,Rodnina, M.V.
Modulation of translational decoding by m 6 A modification of mRNA.
Nat Commun, 14:4784-4784, 2023

PubMed Abstract: N-methyladenosine (mA) is an abundant, dynamic mRNA modification that regulates key steps of cellular mRNA metabolism. mA in the mRNA coding regions inhibits translation elongation. Here, we show how mA modulates decoding in the bacterial translation system using a combination of rapid kinetics, smFRET and single-particle cryo-EM. We show that, while the modification does not impair the initial binding of aminoacyl-tRNA to the ribosome, in the presence of mA fewer ribosomes complete the decoding process due to the lower stability of the complexes and enhanced tRNA drop-off. The mRNA codon adopts a π-stacked codon conformation that is remodeled upon aminoacyl-tRNA binding. mA does not exclude canonical codon-anticodon geometry, but favors alternative more dynamic conformations that are rejected by the ribosome. These results highlight how modifications outside the Watson-Crick edge can still interfere with codon-anticodon base pairing and complex recognition by the ribosome, thereby modulating the translational efficiency of modified mRNAs.

PubMed: 37553384
DOI: 10.1038/s41467-023-40422-7

実験手法

ELECTRON MICROSCOPY (2.88 Å)